FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004718044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614787
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGTTAGCTCACCGTACACTTTTGGCCAGGGGA27280.44373091818792526No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26560.4320195449806193No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGTTAAGAGTGAAGTCTGTTCCAGACCCACTGCC20240.32921971349426715No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT19600.31880960397666186No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC17330.28188624678140556No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14290.23243822657278052No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13970.22723317181397784No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGTCGCAGCTACTTAGCCTGGTACC13870.22560659220185203No Hit
GTCTCCAGGCACCCTGTCTCTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGTCG13820.2247933023957891No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13670.22235343297760038No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA13450.21877495783092357No Hit
GTCTGGAACAGACTTCACTCTTAACATCAGCAGACTGGAGCCTGAAGATT12810.20836484831331828No Hit
ATGTTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA12360.20104524005875204No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGTTAAGAGTGAAGTCTGTTCCAGACCCACTGCC11730.19079778850235937No Hit
GTGTATTACTGTCAGCAGTATGTTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA11660.18965918277387128No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG11570.18819526112295804No Hit
CATCTATGGTGTATCCAACAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGAACAGACTTC11360.1847794439374938No Hit
CTCCAGGCACCCTGTCTCTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGTCGC10930.17778515160535274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG10600.17241743888533753No Hit
GTTCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGTT10550.1716041490792746No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT10260.16688706820410973No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGTGAGCTAACATACTGCTGAC10210.1660737783980468No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10170.16542314655319648No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC10010.16282061917379514No Hit
GAGTATTAGTCGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGT10000.16265796121258258No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG9890.16086872363924415No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG9560.15550101091922894No Hit
GTCTCTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA9440.15354911538467794No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9180.14932000839315082No Hit
GTATGTTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9110.14818140266466273No Hit
TCTATGGTGTATCCAACAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGAACAGACTTCACT9100.14801874470345014No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8870.14427761159556074No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC8830.14362697975071043No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC8690.14134976829373427No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTCTGTCTCC8600.139885846642821No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT8480.13793395110827003No Hit
GAGCTAACATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGTTAAGAGTGAAGT8460.13760863518584485No Hit
GGCCAAAAGTGTACGGTGAGCTAACATACTGCTGACAGTAATACACTGCA8460.13760863518584485No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8430.1371206613022071No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8380.1363073714961442No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCGACTAATACTCTG8270.1345181339228058No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC8170.13289155431067995No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA7980.1298010530476409No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7950.12931307916400314No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7920.1288251052803654No Hit
ATCCAACAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGAACAGACTTCACTCTTAACATC7790.1267105517846018No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGTGAGCT7770.12638523586217665No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC7730.12573460401732633No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGAACAGACTTCACTCTTAA7450.12118018110337402No Hit
GTATCCAACAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGAACAGACTTCACTCTTAACATC7450.12118018110337402No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7330.11922828556882302No Hit
GTATTAGTCGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGTAT7270.11825233780154752No Hit
CCCTGTCTCTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGTCGCAGCTACTT7130.11597512634457137No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT6990.11369791488759522No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6960.11320994100395747No Hit
CTCCCAGGCTCCTCATCTATGGTGTATCCAACAGGGCCGCTGGCATCCCA6880.1119086773142568No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC6860.11158336139183164No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC6770.1101194397409184No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6660.10833020216758No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6540.106378306633029No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6480.10540235886575351No Hit
GCTAACATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTC6430.10458906905969059No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT6380.10377577925362769No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC6230.10133590983543894No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6150.10003464614573827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)