FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097607
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40620.37007781473696866No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT37120.338190262999416No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA27090.24680965044865788No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26030.23715227763671334No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG25340.2308658745798815No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24870.2265838319179816No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23490.21401102580431794No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22970.20927344668902442No Hit
GATCTATGCTGCATCCCATTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGTTCTGGGACAGAATTC22730.20708687171273504No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCGTTAGCAC21840.19897832284232883No Hit
CTGTAACTCGATTGACAGTAATAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC21800.1986138936796139No Hit
GTCTCTGGTGGCTCCATCAGGAGTAACTCCTGGACCTGGATCCGGCAGAC21120.19241859791346083No Hit
ACAGTGTCCTGCTCGCTTTCGGCGGTGGGACCAGGGTGGAGATCAAACGA20420.18604108756595028No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20330.1852211219498418No Hit
GAGTTACAGTGTCCTGCTCGCTTTCGGCGGTGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT19570.1782969678582589No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC19550.17811475327690146No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19210.17501710539382492No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT18550.16900402420902927No Hit
GGACACTGTAACTCGATTGACAGTAATAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAATT17910.16317315760559106No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17750.1617154409547315No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA17440.15889111494369113No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCGTTAGCAC17200.1567045399674018No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCACCGCCGAAAGCGAGCAGGACACTGTAACTCGATTGAC16830.15333357021228908No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC16560.15087367336396362No Hit
GCATAGATCAGGAGCTTAGGGGCTCTCCCTAATTTCTGCTGATACCAATT16350.14896042025971046No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA15920.1450428067605254No Hit
GTTCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTATTACTGTCAATCG15780.14376730469102328No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15670.14276512449355733No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTATTACTGTCAATCGAGTTACAGTGTC15580.14194515887744885No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15400.14030522764523187No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15330.1396674766104808No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGGAGTAACTCCTGGACCTGGATCCGGCAGACCCCAGGGAAGGG15240.13884751099437231No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT15040.13702536518079786No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14370.1309211767053235No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14360.13083006941464476No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14250.12982788921717883No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCACCGCCGAAAGCGAGCAGGAC14250.12982788921717883No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14220.12955456734514265No Hit
GTCCTGCTCGCTTTCGGCGGTGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC13750.1252725246832427No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13740.12518141739256403No Hit
ATGCTGCATCCCATTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGTTCTGGGACAGAATTCACTCTC13700.12481698822984912No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12980.11825726330098113No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12930.11780172684758752No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12710.11579736645265565No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG12670.11543293728994075No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC12620.11497740083654714No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12560.1144307570924748No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12270.11178864566279187No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12040.10969317797718127No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGATCTGGTTCTTGGACGTGTCTC11980.10914653423310892No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11910.10850878319835788No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA11890.10832656861700043No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC11860.10805324674496428No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11860.10805324674496428No Hit
GTCTGCAGCCTGAGGATTTTGCAACTTATTACTGTCAATCGAGTTACAGTGTCCTGCTCGCTTTCGGCGGTGGG11740.10695995925681961No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC11690.106504422803426No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC11670.10632220822206855No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC11440.10422674053645795No Hit
GTGTATTACTGTGCGAGACGGGGCGGTAGTAACTTTATATACTTCTTTGACTACTGGGGCCAGGGAATCCTGGTC11300.10295123846695584No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC11210.10213127285084735No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11040.10058244890930906No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11000.1002180197465942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)