FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097607
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39480.3596915835995944No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT29410.2679465418861214No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA24920.22703936837137517No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24420.2224840038374391No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23370.21291773831617328No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA22020.2006182540745458No Hit
GATCTATGCTGCATCCCATTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGTTCTGGGACAGAATTC21440.19533403121517992No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20870.19014091564649277No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20680.18840987712359705No Hit
CTGTAACTCGATTGACAGTAATAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC20410.18594998027527157No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG19350.17629260746332703No Hit
ACAGTGTCCTGCTCGCTTTCGGCGGTGGGACCAGGGTGGAGATCAAACGA19270.17556374913789727No Hit
GTCTCTGGTGGCTCCATCAGGAGTAACTCCTGGACCTGGATCCGGCAGAC19040.17346828145228665No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCGTTAGCAC18830.1715550283480335No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC18680.17018841898785267No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT18150.1653597325818804No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17520.15961997326912092No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17320.1577978274555465No Hit
GAGTTACAGTGTCCTGCTCGCTTTCGGCGGTGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT17150.1562490035140082No Hit
GGACACTGTAACTCGATTGACAGTAATAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAATT16970.15460907228179122No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT15840.14431394843509562No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15790.14385841198170202No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCGTTAGCAC15690.1429473390749148No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCACCGCCGAAAGCGAGCAGGACACTGTAACTCGATTGAC15630.14240069533084246No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC15460.1408518713893042No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14750.13438325375111493No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG14560.1326522152282192No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTATTACTGTCAATCGAGTTACAGTGTC14340.13064785483328734No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14340.13064785483328734No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC14220.12955456734514265No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14160.12900792360107033No Hit
GCATAGATCAGGAGCTTAGGGGCTCTCCCTAATTTCTGCTGATACCAATT14150.1289168163103916No Hit
GTTCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTATTACTGTCAATCG14070.12818795798496183No Hit
ATGCTGCATCCCATTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGTTCTGGGACAGAATTCACTCTC13940.12700356320613845No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13700.12481698822984912No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGGAGTAACTCCTGGACCTGGATCCGGCAGACCCCAGGGAAGGG13560.123541486160347No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13510.12308594970695338No Hit
GTCCTGCTCGCTTTCGGCGGTGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC13500.12299484241627469No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13480.12281262783491723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13300.12117269660270023No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12970.11816615601030242No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCACCGCCGAAAGCGAGCAGGAC12870.1172550831035152No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12510.11397522063908119No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12270.11178864566279187No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12110.11033092901193231No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12070.10996649984921743No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC11990.10923764152378766No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC11910.10850878319835788No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11740.10695995925681961No Hit
CTCCTGATCTATGCTGCATCCCATTTGCAAAGTGGGGTCCCATCGAGGTT11740.10695995925681961No Hit
GTCTGCAGCCTGAGGATTTTGCAACTTATTACTGTCAATCGAGTTACAGTGTCCTGCTCGCTTTCGGCGGTGGG11700.10659553009410472No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA11670.10632220822206855No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGATCTGGTTCTTGGACGTGTCTC11530.10504670615256644No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11310.10304234575763457No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11280.1027690238855984No Hit
GTCCCAGAACCACTGCCACTGAACCTCGATGGGACCCCACTTTGCAAATGGGATGCAGCATAGATCAGGAGCTT11240.1024045947228835No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC11200.10204016556016862No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11080.10094687807202396No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC11050.1006735561999878No Hit
GAGTGAATTCTGTCCCAGAACCACTGCCACTGAACCTCGATGGGACCCCACTTTGCAAATGGGATGCAGCAT11050.1006735561999878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)