FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004718056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780359
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGGTTGCTGCCTGCAAAGGAGCTGCAGTAATAAT24030.30793519392997326No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21440.2747453415671505No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20930.26820988801308115No Hit
GTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGAGACAGTGAGTTCTTGGCGTTGTCTCTGG20390.2612899960146548No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGGTTGCTGCCTGCAAAGGAGCTG20060.2570611731267276No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA19840.25424195786810944No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGTCAGTCAGTCACCATCTCCTG19540.2503975734245392No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19190.245912458240374No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15890.20362422936110175No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC15740.20170203713931667No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCCTTT15720.20144574484307864No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15170.19439770669653325No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT15070.1931162452153432No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14940.1914503452897961No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14800.18965629921613No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14330.1836334302545367No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC13860.1776105612929434No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGTCAGTCAGTCAC13820.17709797670046734No Hit
GATTATTACTGCAGCTCCTTTGCAGGCAGCAACCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT13810.17696983055234833No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13510.17312544610877814No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC12700.16274560811113858No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC12500.16018268514875847No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG12160.15582571611271223No Hit
GTGTATTACTGTGCGAGAGATCAAGGTTATTACCGCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC12130.15544127766835522No Hit
GGTATCAACAGCACCCAGGCAAAGCCCCCAAACTCATCCTTTATGAGGTCACTAAGCGGCCCTCAGGGGTCCCTG12060.15454425463152216No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT12030.15415981618716515No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11930.15287835470597508No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG11790.15108430863230898No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC11280.1445488550782396No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGGTTGCTGCCTGCAAAGGAGCTGCAGTAATAATC11040.14147334752338347No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC10960.14044817833843143No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10800.1383978399685273No Hit
ACCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC10520.13480974782119512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10400.13327199404376705No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10380.13301570174752902No Hit
GGTTATGACTATGTCTCCTGGTATCAACAGCACCCAGGCAAAGCCCCCAAACTCATCCTTTATGAGGTCACTA10380.13301570174752902No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10160.13019648648891088No Hit
TCCTTTATGAGGTCACTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCT9740.12481434826791257No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA9740.12481434826791257No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA9630.12340474063860352No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAAGGATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGATACCAGG9560.12250771760177047No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9510.12186698686117543No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGTCAGTCAGTCACCATCTCCTGCGCTGGAACCA9490.12161069456493741No Hit
GTTGATACCAGGAGACATAGTCATAACCACCAACGTCACTGTTGGTTCCAGCGCAGGAGATGGTGACTGACTGAC9470.1213544022686994No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9450.12109810997246138No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9370.12007294078750932No Hit
CTCCTGGTCAGTCAGTCACCATCTCCTGCGCTGGAACCAACAGTGACGTTGGTGGTTATGACTATGTCTCCTGGT9330.11956035619503332No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC9300.11917591775067629No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9300.11917591775067629No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT9220.11815074856572423No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG9180.11763816397324821No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGTC9120.11686928708453416No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT9090.11648484864017715No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8940.11456265641839206No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT8830.11315304878908297No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8740.11199973345601191No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8640.11071827197482184No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC8560.1096931027898698No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGTCAGTCAGTCAC8490.10879607975303675No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8430.10802720286432271No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC8420.10789905671620369No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC8310.10648944908689462No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA8220.10533613375382357No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC8210.10520798760570454No Hit
CTCCTGCGCTGGAACCAACAGTGACGTTGGTGGTTATGACTATGTCTCCTGGTATCAACAGCACCCAGGCAAAGC8180.10482354916134753No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8130.1041828184207525No Hit
GTCAGTCACCATCTCCTGCGCTGGAACCAACAGTGACGTTGGTGGTTATGACTATGTCTCCTGGTATCAACAGC7950.10187618775461037No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7810.10008214168094429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)