FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004718059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420490
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA14890.3541106803966801No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11150.26516682917548573No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT10070.23948250850198577No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTTGCTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGGCTGGAGTGG9150.21760327237270805No Hit
GTGTATTACTGTGCGAGACGCGGGGGGAGCCTACCCCGAAAACAGTACTTCTTTGACTACTGGGGCCAGGGAACC8920.2121334633403886No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8700.20690147209208304No Hit
CCTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTG8610.20476111203595804No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7850.18668696045090252No Hit
ACAGTACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA7840.1864491426668886No Hit
GAGTTACAGTACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT7750.18430878261076364No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7430.1766986135223192No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7400.17598516017027752No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCT7330.1743204356821803No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7280.17313134676211087No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7240.1721800756260553No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6970.16575899545768033No Hit
GGGTACTGTAACTCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT6900.1640942709695831No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC6820.16219172869747198No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG6490.15434374182501368No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5980.14221503484030537No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTTTCAGCAGAA5900.14031249256819425No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG5890.14007467478418037No Hit
GTATTACTGTGCGAGACGCGGGGGGAGCCTACCCCGAAAACAGTACTTCTTTGACTACTGGGGCCAGGGAACC5580.1327023234797498No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG5530.13151323455968036No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5530.13151323455968036No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5470.13008632785559704No Hit
CTGTAACTCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC5450.12961069228756927No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5370.12770815001545816No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG5270.12532997217531927No Hit
ATCTATCATAGTGGGAGCACCTCCTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCA5260.1250921543913054No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5250.1248543366072915No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTC5240.1246165188232776No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG5180.12318961211919427No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGC5170.12295179433518039No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG5170.12295179433518039No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT5110.12152488763109706No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC5100.12128706984708318No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC5040.11986016314299984No Hit
ATCATAGTGGGAGCACCTCCTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACC5010.11914670979095816No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC4860.11557944303074984No Hit
GTCCCTCACCTGCGCTGTCTCTGGTTACTCCATCAGCAGTGGTTGCTACT4830.11486598967870816No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT4770.11343908297462484No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4730.11248781183856929No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGGGT4700.11177435848652763No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGTCCCAGTCTCCATCCTCCCTGTCTGCATCTGT4690.11153654070251375No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA4560.10844490951033318No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4530.10773145615829152No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT4510.10725582059026373No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG4510.10725582059026373No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC4490.10678018502223596No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGAAGTACTGTTTTCGGGGTAGGCTCCCCCCGCGTCTCGCACAGTAATAC4470.10630454945420818No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG4470.10630454945420818No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC4470.10630454945420818No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC4420.10511546053413874No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA4350.10345073604604152No Hit
GCATAGATCACGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGAAACCAATT4280.1017860115579443No Hit
GGCCAAAAGTGTACGGGGTACTGTAACTCTGTTGACAGTAATAAGTTGCA4280.1017860115579443No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGGGTACTGTAACTCTGTTGAC4270.10154819377393041No Hit
CCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGT4270.10154819377393041No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC4230.10059692263787487No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4220.10035910485386096No Hit
GTACTGTAACTCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT4210.10012128706984708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)