FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004718062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874565
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGG36190.41380572055822035No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC21720.2483520378702555No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC20470.23405921801123988No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGGGATGGTTACCACTGCTGTCCCGGGAGTTACAGT20260.23165802427492524No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19330.22102416629981764No Hit
AGCCAGGACAGGCCCCTGTACTTGTCTTCTATGGTAAAAACAACCGGCCC18750.21439229788523437No Hit
GTGTATTACTGTGCGAGAGTCCATAGCAGTGGCTGGTACGATCTTCTTGACTACTGGGGCCAGGGAACCCTGGTC18160.207646086911779No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17750.20295804199802187No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG17490.19998513546734664No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17350.19838433964313684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17200.196669201260055No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA16490.1885508795801341No Hit
CCCCTGTACTTGTCTTCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA16380.18729311143254074No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16120.1843202049018655No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGA15890.18169032604780663No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15840.181118613253446No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATT15010.17162818086705964No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14890.17025607016059413No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGG14870.17002738504284987No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAACATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG14570.16659710827668614No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14380.16442459965811576No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14160.161909063362929No Hit
GGTTACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGG14130.16156603568631261No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGTTATAGCTACTGAAGGTGAATCCAGAGGCTGCAC13430.15356205656526387No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTT13200.15093217771120498No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13110.14990309468135588No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA13070.14944572444586737No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12800.14635847535632No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12730.14555807744421512No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12420.14201345811917926No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12410.14189911556030713No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGG12390.1416704304425629No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT12300.14064134741271375No Hit
GTGGTAACCATCCCGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA12130.13869752391188764No Hit
GTATTACTGTGCGAGAGTCCATAGCAGTGGCTGGTACGATCTTCTTGACTACTGGGGCCAGGGAACCCTGGTCAC11990.13709672808767787No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT11840.13538158970459602No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11730.13412382155700261No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11730.13412382155700261No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGT11650.13320908108602564No Hit
GGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATCCCGTGGTATTCGGCGGAGGGACCA11490.1313796001440716No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11470.1311509150263274No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11340.12966446176098975No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCC11240.1285210361722685No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT11010.12589115731820963No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10950.12520510196497686No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10930.12497641684723261No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG10930.12497641684723261No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10830.12383299125851138No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10740.12280390822866225No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10640.121660482639941No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC10610.12131745496332463No Hit
GTATATGTAACTACTACTACTACTAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT10540.12051705705121976No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10250.11720112284392813No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10190.11651506749069537No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC10140.11594335469633475No Hit
GTGTATTACTGTGCGAGAGTCCATAGCAGTGGCTGGTACGATCTTCTTGACTACTGGGGCCAGGGAACCCTGGT10060.11502861422535776No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA10000.114342558872125No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC10000.114342558872125No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGG10000.114342558872125No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9940.11365650351889225No Hit
GCTATGGACTCTCGCACAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTT9880.1129704481656595No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9860.11274176304791525No Hit
GGGTTCCCTGGCCCCAGTAGTCAAGAAGATCGTACCAGCCACTGCTATGG9860.11274176304791525No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9850.11262742048904313No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9820.11228439281242675No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAAC9670.11056925442934487No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTG9530.10896845860513511No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9450.10805371813415812No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGT9390.10736766278092538No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG9370.10713897766318112No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC9350.10691029254543687No Hit
GTCCATAGCAGTGGCTGGTACGATCTTCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCC9280.106109894633332No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG9260.10588120951558774No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9180.10496646904461075No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTG9150.10462344136799437No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAAGATCGTACC9020.10313698810265676No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGGGATGGTTACCACTGCTGTCCCGGGAGTTACAGTA8860.10130750716070276No Hit
GCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGCTGGAGCCAGAGAATCGGT8810.10073579436634213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)