FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569457
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT52790.33635837107993405No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC51740.3296681591149041No Hit
GACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACAAAGAGGGCACTCAGGCTGCTATCCCATGTTCCGCAGT45360.2890171568892936No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44330.28245437753312136No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC43520.27729335687438394No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCC41910.26703503186133803No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40610.2587519122855867No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC39000.24849358727254078No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG36470.23237336225204003No Hit
ACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACAAAGAGGGCACTCAGGCTGCTATCCCATGTTCCGCAGTA34530.22001239919284188No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG33760.2151062437518199No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31920.20338244373691028No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG28870.183948970886109No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT28410.18101802088238161No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACAAA28140.1792976806628025No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG28050.1787242339229428No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG27330.17413666000406508No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC26780.17063226326047798No Hit
GTTCCTGGGAGCTGCTGGTACCAGGCCACATAATTATTACCAATGTTGGAGCTGCCTCCAGAGCAGGAGATGGTG26400.16821104369218143No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24740.15763413715699123No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAG23620.1504979110609593No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC23540.14998818062552846No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23300.1484589893192359No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCCCTCTTTGTCTTCGGATCTGGGACCAAGGTCACCGTC23200.14782182627494733No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAGGCAGCTCCAACATTGGTAATAATTATGTGGCCTGGTACCAGC22320.14221479148520794No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21750.1385829621327631No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACAAAGAGGGC21710.1383280969150477No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21230.13526971430246257No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCCCTCTTTGTCTTCGG20650.13157416864558888No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG20250.1290255164684346No Hit
GTTTGGGGGCTGTTCCTGGGAGCTGCTGGTACCAGGCCACATAATTATTACCAATGTTGGAGCTGCCTCCAGAGC20100.12806977190200178No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG19990.12736889255328435No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA19890.12673172950899578No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG19620.12501138928941666No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG19540.1245016588539858No Hit
ACATGGGATAGCAGCCTGAGTGCCCTCTTTGTCTTCGGATCTGGGACCAA19520.12437422624512809No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG19180.12220787189454697No Hit
CTCCTGCTCTGGAGGCAGCTCCAACATTGGTAATAATTATGTGGCCTGGTACCAGCAGCTCCCAGGAACAGCCCC18740.11940435449967729No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA18530.11806631210667129No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18290.11653712080037873No Hit
GCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT18140.11558137623394588No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG17850.11373360340550905No Hit
CAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGC17520.11163096535935678No Hit
GGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCT17490.11143981644607019No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17450.11118495122835477No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17440.11112123492392591No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT17410.11093008601063936No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC17380.11073893709735277No Hit
GGATAGCAGCCTGAGTGCCCTCTTTGTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCA17380.11073893709735277No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG17370.11067522079292393No Hit
GCTGGTACCAGGCCACATAATTATTACCAATGTTGGAGCTGCCTCCAGAG17140.10920974579106023No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGA17110.10901859687777364No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16930.10787170339805421No Hit
ACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTG16670.10621507948290396No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCC16290.1037938599146074No Hit
GTCTCTGGTGACTCCATGATTGGTTACTACTGGAACTGGATCCGGCAGCC16020.10207351969502827No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCCCT15950.10162750556402629No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15930.10150007295516857No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG15810.1007354773020223No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15780.10054432838873571No Hit
GTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTC15750.10035317947544917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)