FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004718069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572853
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT55270.35139965400453826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49070.3119808399132023No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC37960.24134486821082451No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT37940.24121771074601378No Hit
GTCTGCATCTGTGGGTGACAGGGTCACCATCACTTGCCGGGCCAGTCAGAGCATTAGTACTTGGTTGGCCTGGTA34840.2215083037003458No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33680.21413317074132168No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33260.21146286398029565No Hit
GAACTGGACTGCTGGCAGTAATAAGTTGCAAAATCATCAGGCTGTAGGCTGTTGATGGTGAGTGTGAATTCTGTC32890.2091104508812966No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32660.20764814003597282No Hit
GTCCAGTTCTTATCCGTACAGTTTTGGCCAGGGGACCAAGTTGGAGATCAGACGAACTGTGGCTGCACCA32580.2071395101767298No Hit
CCTTCCACCCTGTCTGCATCTGTGGGTGACAGGGTCACCATCACTTGCCGGGCCAGTCAGAGCATTAGTACTTGG31590.20084521566859714No Hit
GAATTCACACTCACCATCAACAGCCTACAGCCTGATGATTTTGCAACTTATTACTGCCAGCAGTCCAGTTCTTA31490.2002094283445433No Hit
GTTTTGGCCAGGGGACCAAGTTGGAGATCAGACGAACTGTGGCTGCACCA31270.19881069623162495No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30830.19601323200578818No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC30780.19569533834376132No Hit
GGCCAAAACTGTACGGATAAGAACTGGACTGCTGGCAGTAATAAGTTGCAAAATCATCAGGCTGTAGGCTGTTGA30400.19327934651235684No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28870.1835518004543336No Hit
GATAAGAACTGGACTGCTGGCAGTAATAAGTTGCAAAATCATCAGGCTGTAGGCTGTTGATGGTGAGTGTGAAT28050.17833834439709242No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27150.17261625848060816No Hit
GTTCTTATCCGTACAGTTTTGGCCAGGGGACCAAGTTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCA24610.15646726044964152No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23890.1518895917164541No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23670.15049085960353575No Hit
CACCATCAACAGCCTACAGCCTGATGATTTTGCAACTTATTACTGCCAGCAGTCCAGTTCTTATCCGTACAGTTT23520.14953717861745502No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA23220.14762981664529362No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAACTTGGTCCCCTGGCCAAAACTG23140.14712118678605057No Hit
GCCTACAGCCTGATGATTTTGCAACTTATTACTGCCAGCAGTCCAGTTCTTATCCGTACAGTTTTGGCCAGGGGA22950.14591319087034835No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22880.14546813974351067No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22560.1434336203065385No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22350.1420984669260255No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21220.1349140701642175No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20330.12925556298013863No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20050.127475358472788No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG19770.12569515396543735No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAACTTGGTCC19740.1255044177682212No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19680.1251229453737889No Hit
ATCTCATACAGTTCAGCTGATAAAATGTACGCAGATTCCGTCAAGGGCCG19650.12493220917657276No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC19440.12359705579605977No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG19320.1228341110071952No Hit
TCTCTAAGGCGTCAATGTTACAAACTGGGGTCCCAGCAACCTTCAGCGGCAGTGGATCTGGGACAGAATTCACA18350.11666697396387331No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18340.11660339523146791No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG18080.11495034818892803No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17810.11323372241398273No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17550.11158067537144285No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG17520.11138993917422671No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17310.11005478579371372No Hit
GTATCAGCACAAACCGGGGAAAGCCCCTAAACTCCTAATCTCTAAGGCGT17190.10929184100484915No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT17190.10929184100484915No Hit
GCATTAGTACTTGGTTGGCCTGGTATCAGCACAAACCGGGGAAAGCCCCTAAACTCCTAATCTCTAAGGCGTCAA17090.10865605368079534No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGTGACAGGGTCACCATCACTTGCCGGGCCAGTCAGAGCATTAGTA17040.10833816001876845No Hit
GAGCATTAGTACTTGGTTGGCCTGGTATCAGCACAAACCGGGGAAAGCCC16790.10674869170863392No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16670.10598574691976936No Hit
GTGTGAATTCTGTCCCAGATCCACTGCCGCTGAAGGTTGCTGGGACCCCAGTTTGTAACATTGACGCCTTAGAGA16470.10471417227166176No Hit
GTTGATGGTGAGTGTGAATTCTGTCCCAGATCCACTGCCGCTGAAGGTTGCTGGGACCCCAGTTTGTAACATTGA16450.104587014806851No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16080.10223460170785191No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGCTGACCCAGTCTCCTTCCACC15810.10051797593290664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)