FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004718070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572853
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56010.35610448020253643No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA44760.2845784062464833No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37340.23740298680169095No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT34940.22214409102439958No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33690.21419674947372705No Hit
GAACTGGACTGCTGGCAGTAATAAGTTGCAAAATCATCAGGCTGTAGGCTGTTGATGGTGAGTGTGAATTCTGTC31670.2013538455278402No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC30940.1967125980622474No Hit
GTCTGCATCTGTGGGTGACAGGGTCACCATCACTTGCCGGGCCAGTCAGA30930.19664901932984202No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29710.1888924139763856No Hit
GTCCAGTTCTTATCCGTACAGTTTTGGCCAGGGGACCAAGTTGGAGATCAGACGAACTGTGGCTGCACCAT29550.1878751542578995No Hit
GGCCAAAACTGTACGGATAAGAACTGGACTGCTGGCAGTAATAAGTTGCAAAATCATCAGGCTGTAGGCTGTT29250.1859677922857381No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA28580.1817080172145776No Hit
GAATTCACACTCACCATCAACAGCCTACAGCCTGATGATTTTGCAACTTATTACTGCCAGCAGTCCAGTTCTTAT28340.18018212763684846No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC28010.1780840294674709No Hit
GTTTTGGCCAGGGGACCAAGTTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT27840.17700319101657944No Hit
GTGTGAATTCTGTCCCAGATCCACTGCCGCTGAAGGTTGCTGGGACCCCAGTTTGTAACATTGACGCCTTAGA27640.17573161636847182No Hit
CCTTCCACCCTGTCTGCATCTGTGGGTGACAGGGTCACCATCACTTGCCG26770.1702002666492037No Hit
GATAAGAACTGGACTGCTGGCAGTAATAAGTTGCAAAATCATCAGGCTGTAGGCTGTTGATGGTGAGTGTGAATT26530.1686743770714746No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25750.1637152359438549No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25700.16339734228182798No Hit
GCCTACAGCCTGATGATTTTGCAACTTATTACTGCCAGCAGTCCAGTTCTTATCCGTACAGTTTTGGCCAGGG24530.15595863059039847No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA24340.15475063467469624No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAACTTGGTCCCCTGGCCAAAACTG24240.15411484735064243No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC24150.15354263875899402No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23390.14871065509618508No Hit
GTTGATGGTGAGTGTGAATTCTGTCCCAGATCCACTGCCGCTGAAGGTTGCTGGGACCCCAGTTTGTAACATT23280.1480112890397259No Hit
GTTCTTATCCGTACAGTTTTGGCCAGGGGACCAAGTTGGAGATCAGACGA23230.14769339537769902No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22430.14260709678526856No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA21730.13815658551689192No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21730.13815658551689192No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21490.13663069593916277No Hit
CACCATCAACAGCCTACAGCCTGATGATTTTGCAACTTATTACTGCCAGC20980.13338818058648838No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20610.1310357674874893No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18920.12029096171097997No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAACTTGGTCC18560.11800212734438628No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18540.11787496987957552No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA18540.11787496987957552No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGT18500.117620654949954No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18410.11704844635830557No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18190.11564971424538721No Hit
ATCTCATACAGTTCAGCTGATAAAATGTACGCAGATTCCGTCAAGGGCCG18130.11526824185095493No Hit
TCTCTAAGGCGTCAATGTTACAAACTGGGGTCCCAGCAACCTTCAGCGGCAGTGGATCTGGGACAGAATTCACA18090.1150139269213334No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17790.11310656494917198No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGT17340.11024552199092987No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT17030.10827458128636305No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16990.10802026635674154No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15960.10147165691898734No Hit
GTATCAGCACAAACCGGGGAAAGCCCCTAAACTCCTAATCTCTAAGGCGT15810.10051797593290664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)