FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986284
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38390.3892387993721889No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT34050.3452352466429548No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC28230.2862258740890048No Hit
GTCTGGGACAGACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATT26100.264629660422353No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAAGACCAGTCGGAGTGTTAGC24540.2488127152017066No Hit
ATGCTACGTCACCTCAGGGGATGTACACATTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGGCTGCAC23790.24120841461485737No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23100.2342124580749561No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22770.23086656581674242No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG21290.21586074599202662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20890.21180511901237373No Hit
CTCCTGCAAGACCAGTCGGAGTGTTAGCAGCACCTGGTTATCCTGGTACC19690.19963823807341496No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG19250.19517704839579675No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAAGACCAGTCGGAGTGTTAGCAGC19100.1936561882784269No Hit
ACGTAGCATAGTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGT19000.19264228153351368No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT18880.1914255934396178No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17610.17854897777921977No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT16800.1703363331454226No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA16800.1703363331454226No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC16690.16922103572601807No Hit
GTGTTAGCAGCACCTGGTTATCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCAT15950.16171812581366016No Hit
CATTTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC15850.16070421906874693No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG15740.15958892164934238No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC14680.14884151015326214No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAAGACCAGTCGGA14600.14803038475733157No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14560.14762482205936625No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14410.14610396194199643No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA14340.14539422722055717No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCCTGGTCCCCTGGCCAAATGTGTACATCCCCTGAGGTGACGTAGCAT13910.14103442821743029No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13370.13555933179489882No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13230.1341398623520203No Hit
ACACTATGCTACGTCACCTCAGGGGATGTACACATTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGG13180.1336329089795637No Hit
GGCCAAATGTGTACATCCCCTGAGGTGACGTAGCATAGTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCC12860.13038840739584134No Hit
CTCCCAGGCTCCTCATTTATGGTGCATCCAGCAGGGCCACTGGCATCCCA12650.12825920323152357No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC12350.12521748299678387No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGATAACCAGGTGCTGCTAACACTCCG12270.1244063576008533No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12130.12298688815797479No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12120.12288549748348346No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC12060.12227715343653553No Hit
CATTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG11990.12156741871509626No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11910.12075629331916567No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11840.12004655859772641No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGATAACCAGGTGCTGCTAACACTCCGACTGGTCTTGCAGG11830.11994516792323509No Hit
GTATTACTGTGTGAGACGTCGGCTGTCTCACTATGTTAGCAATGCTTTCAACTTTGACCACTGGGGCCAGGGAAC11780.11943821455077848No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCC11740.11903265185281318No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC11540.11700483836298671No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT11530.11690344768849541No Hit
GCATAGTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGTCTGTCCC11530.11690344768849541No Hit
GACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACACTATGCTACGTC11510.11670066633951276No Hit
ACCATATACAGTCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAA11440.1159909316180735No Hit
ATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCATC11350.1150784155476516No Hit
GTTTATTACTGTCAACACTATGCTACGTCACCTCAGGGGATGTACACATTTGGCCAGGGGACCAGGCTGGAGATC11300.11457146217519498No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCCTGGTCCCCTGGCCAAATGTGTACATCCCCTG11270.114267290151721No Hit
GTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCC11030.11183391396392926No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11020.11173252328943793No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC10810.10960331912512014No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10330.10473656674953664No Hit
GTACACATTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC10250.10392544135360605No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10240.10382405067911474No Hit
CCTCAGGGGATGTACACATTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTC10210.10351987865564076No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC10100.10240458123623622No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10060.10199901853827092No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC10060.10199901853827092No Hit
TGGTTATCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCAGCAGGGCC9970.10108650246784903No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAT9960.1009851117933577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)