FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986284
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38110.3863998604864319No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT28320.2871383901594267No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC25900.26260184693252653No Hit
GTCTGGGACAGACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATT24850.25195582611093764No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAAGACCAGTCGGAGTGTTAGC22810.23127212851470771No Hit
ATGCTACGTCACCTCAGGGGATGTACACATTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGGCTGCAC22180.22488451602175438No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21340.21636769936448325No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21200.21494822992160473No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA20160.20440359977450714No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19870.20146327021425878No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAAGACCAGTCGGAGTGTTAGCAGC19240.19507565772130542No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT19060.1932506255804616No Hit
CTCCTGCAAGACCAGTCGGAGTGTTAGCAGCACCTGGTTATCCTGGTACC18840.1910200307416525No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG17780.18027261924557228No Hit
ACGTAGCATAGTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGT17040.17276970933321437No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15900.16121117244120353No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15850.16070421906874693No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15560.15776388950849857No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG15530.15745971748502458No Hit
GTGTTAGCAGCACCTGGTTATCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCAT15250.15462077859926757No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14680.14884151015326214No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC14420.14620535261648776No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC14140.1433664137307307No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCCTGGTCCCCTGGCCAAATGTGTACATCCCCTGAGGTGACGTAGCAT14140.1433664137307307No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG14060.1425552883348001No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13980.14174416293886954No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAAGACCAGTCGGA13920.1411358188919216No Hit
CATTTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC13720.13910800540209514No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13690.13880383337862118No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12960.1314023141407546No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12450.12623138974169712No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12370.12542026434576653No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12320.12491331097330993No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC12280.12450774827534462No Hit
GGCCAAATGTGTACATCCCCTGAGGTGACGTAGCATAGTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCC12220.1238994042283967No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT12210.12379801355390535No Hit
CTCCCAGGCTCCTCATTTATGGTGCATCCAGCAGGGCCACTGGCATCCCA12030.12197298141306155No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11890.12055351197018302No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11540.11700483836298671No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGATAACCAGGTGCTGCTAACACTCCGACTGGTCTTGCAGG11490.11649788499053013No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCC11490.11649788499053013No Hit
GACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACACTATGCTACGTC11490.11649788499053013No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCCTGGTCCCCTGGCCAAATGTGTACATCCCCTG11380.11538258757112554No Hit
ACACTATGCTACGTCACCTCAGGGGATGTACACATTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGG11270.114267290151721No Hit
GTTTATTACTGTCAACACTATGCTACGTCACCTCAGGGGATGTACACATTTGGCCAGGGGACCAGGCTGGAGATC11140.11294921138333382No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11070.11223947666189454No Hit
CATTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG10980.11132696059147265No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA10950.11102278856799867No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGATAACCAGGTGCTGCTAACACTCCG10810.10960331912512014No Hit
GCATAGTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGTCTGTCCC10770.10919775642715486No Hit
GTATTACTGTGTGAGACGTCGGCTGTCTCACTATGTTAGCAATGCTTTCAACTTTGACCACTGGGGCCAGGGAAC10700.1084880217057156No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC10450.10595325484343253No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC10420.10564908281995855No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA10410.10554769214546722No Hit
ACCATATACAGTCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAA10320.10463517607504531No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT10260.10402683202809739No Hit
TGGTTATCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCAGCAGGGCC10030.10169484651479696No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC9890.10027537707191843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)