FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559076
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTACTGTGCGACAAATAACTTGAACGGAGAACTTTCCTACTACTT64160.41152580117967313No Hit
GTAGTAGAGAGCTGAGTCCTCGGCCCGCAGGTTGGCCATCTGAAGAAAAACCGTGTTCTTGGTCTTGTCTCTGGA59800.3835605191793088No Hit
GTCCATCACCATCTCCTGCACTGCAACCGGCGGTGTCACTTATATTCATCGTTATGTCTCCTGGTATCAACACC55530.3561725021743648No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51870.33269705902727No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT50990.3270526901831598No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC47480.3045393553617656No Hit
CTCCTGCACTGCAACCGGCGGTGTCACTTATATTCATCGTTATGTCTCCTGGTATCAACACCACCCAGGCAAAGT41700.2674661145447688No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC39700.25463800353542737No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCGTAGTCCC39490.25329105187944656No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39050.25046886745739144No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34070.21852687104413127No Hit
GCCTTGGGCTGACCCAGGACGGTGACCGTAGTCCCACTTCCAAAAACAAAAGGGCTGCCGTCTCTATATGAGCT31130.1996695478603994No Hit
CATATAGAGACGGCAGCCCTTTTGTTTTTGGAAGTGGGACTACGGTCACCGTCCTGGGTCAGCCCAAGGCCAACC30950.19851501786955864No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC30400.19498728734198975No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29890.19171611903460767No Hit
GTATCAACACCACCCAGGCAAAGTCCCCAAACTCCTTATTTTTGATGTCA29880.19165197847956097No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC29020.18613589074554415No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG27490.17632238582339796No Hit
GTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCGCCTGGGCAGTCCATCACCATCTCCTG27350.17542441805274406No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT26300.1686896597728398No Hit
ACCCAGGACGGTGACCGTAGTCCCACTTCCAAAAACAAAAGGGCTGCCGTCTCTATATGAGCTACAATAATAATC25230.16182662038284215No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT24930.15990240373144093No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG24610.1578499059699463No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24610.1578499059699463No Hit
CTCTATATGAGCTACAATAATAATCAGCCTCGTCTTCAGCCTGGAGACCAGAGATGGTCAGGGAGGCCGTGTTGC24290.15579740820845167No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC24210.155284283768078No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23780.15252623990106962No Hit
ATACTATAGCGACTCCGTGAGGGCCAGATTCACCATTTCCAGAGACAAGA23130.14835710382303363No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCGCCTGGGCAGTCCATCAC22600.14495765440555816No Hit
TCTCTGGTCTCCAGGCTGAAGACGAGGCTGATTATTATTGTAGCTCATAT22370.1434824216394839No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22240.14264859442387673No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22060.14149406443303597No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21600.13854359890088747No Hit
GGCTCAGCCTGCCTCCGTGTCTGGGTCGCCTGGGCAGTCCATCACCATCTCCTGCACTGCAACCGGCGGTGTC21560.13828703668070064No Hit
GACCCAGGACGGTGACCGTAGTCCCACTTCCAAAAACAAAAGGGCTGCCGTCTCTATATGAGCTACAATAATAAT20730.13296337061182392No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG20670.13257852728154368No Hit
CTACTACTGTGCGACAAATAACTTGAACGGAGAACTTTCCTACTACTTTGACCACTGGGGCCAGGGAACCCTGGT20450.13116743507051612No Hit
ACCATGGCCTGGGCTCTGCTTTTCCTCGCCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCT19300.1237912712401448No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19040.12212361680893041No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19030.12205947625388372No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18800.12058424348780944No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18450.11833932406117469No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18360.11776205906575433No Hit
CAGTAGTAGAGAGCTGAGTCCTCGGCCCGCAGGTTGGCCATCTGAAGAAA18180.1166075290749136No Hit
CTTATATTCATCGTTATGTCTCCTGGTATCAACACCACCCAGGCAAAGTCCCCAAACTCCTTATTTTTGATGTC18180.1166075290749136No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18170.1165433885198669No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG18160.11647924796482018No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18000.11545299908407287No Hit
GGCCTGGGCTCTGCTTTTCCTCGCCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGC17970.11526057741893275No Hit
TCTCTGGATTGTCCGTCAGTGACTACTCGATAAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCT17900.1148115935336058No Hit
GTATAACGTCGGTGACACATACTATAGCGACTCCGTGAGGGCCAGATTCACCATTTCCAGAGACAAGACCAAGA17770.1139777663179986No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17730.11372120409781179No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCGCCTGGG17570.11269495521706446No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16980.10891066246930875No Hit
CTATAGTATGTGTCACCGACGTTATACAGAACTGAGACCCACTCCAGCCC16950.10871824080416863No Hit
GTGTTGATACCAGGAGACATAACGATGAATATAAGTGACACCGCCGGTTGCAGTGCAGGAGATGGTGATGGACTG16910.1084616785839818No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTTTTCCTCGCCCTCCTCACTCAGGGCACAGGGTCCTGGGC16900.10839753802893508No Hit
CTATATGAGCTACAATAATAATCAGCCTCGTCTTCAGCCTGGAGACCAGA16710.10717886748304765No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCGTAGTCCCACTTCCAAAAACAAAAGGGCTGC16630.106665743042674No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA16550.10615261860230034No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16480.10570363471697339No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA16440.10544707249678656No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG16320.10467738583622609No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGATCTTCCTTGT16280.10442082361603924No Hit
GTTCTGTATAACGTCGGTGACACATACTATAGCGACTCCGTGAGGGCCAGATTCACCATTTCCAGAGACAAGACC16220.10403598028575899No Hit
GCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCGCCTGGGCAGTCCATCAC16090.1032021530701518No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGA15970.10243246640959133No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15910.10204762307931108No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC15850.10166277974903083No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG15800.1013420769737973No Hit
CACTTATATTCATCGTTATGTCTCCTGGTATCAACACCACCCAGGCAAAGTCCCCAAACTCCTTATTTTTGATGT15730.10089309308847035No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15660.1004441092031434No Hit
GATTATTATTGTAGCTCATATAGAGACGGCAGCCCTTTTGTTTTTGGAAGTGGGACTACGGTCACCGTCCTGGGT15650.1003799686480967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)