FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004718082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559076
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTACTGTGCGACAAATAACTTGAACGGAGAACTTTCCTACTACTT60180.3859978602710837No Hit
GTCCATCACCATCTCCTGCACTGCAACCGGCGGTGTCACTTATATTCATCGTTATGTCTCCTGGTATCAACACC52230.33500611900895144No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT46770.2999853759534494No Hit
GTAGTAGAGAGCTGAGTCCTCGGCCCGCAGGTTGGCCATCTGAAGAAAAACCGTGTTCTTGGTCTTGTCTCTGGA45280.29042843325149No Hit
CTCCTGCACTGCAACCGGCGGTGTCACTTATATTCATCGTTATGTCTCCTGGTATCAACACCACCCAGGCAAAGT40260.258229874618043No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40200.25784503128776276No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCGTAGTCCC38520.24706941803991594No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38460.2466845747096357No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC36590.23469029091590146No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC35680.2288535004066511No Hit
GCCTTGGGCTGACCCAGGACGGTGACCGTAGTCCCACTTCCAAAAACAAAAGGGCTGCCGTCTCTATATGAGCT33410.2142935944110486No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32660.20948305278254553No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31270.2005675156310533No Hit
GTATCAACACCACCCAGGCAAAGTCCCCAAACTCCTTATTTTTGATGTCA28660.1838268307638627No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT28500.18280058188311538No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC27800.1783107430298459No Hit
ACCCAGGACGGTGACCGTAGTCCCACTTCCAAAAACAAAAGGGCTGCCGTCTCTATATGAGCTACAATAATAATC27770.17811832136470576No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG27700.17766933747937882No Hit
CATATAGAGACGGCAGCCCTTTTGTTTTTGGAAGTGGGACTACGGTCACCGTCCTGGGTCAGCCCAAGGCCAACC27440.17600168304816444No Hit
GTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCGCCTGGGCAGTCCATCACCATCTCCTG27040.17343606084629615No Hit
CTCTATATGAGCTACAATAATAATCAGCCTCGTCTTCAGCCTGGAGACCAGAGATGGTCAGGGAGGCCGTGTTGC25000.16035138761676787No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24140.15483529988275108No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC23530.15092272602490192No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCGCCTGGGCAGTCCATCAC23080.1480364010478001No Hit
GACCCAGGACGGTGACCGTAGTCCCACTTCCAAAAACAAAAGGGCTGCCGTCTCTATATGAGCTACAATAATAAT22230.14258445386883No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG21610.13860773945593416No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21300.13661938224948622No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21270.1364269605843461No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20960.1344386033778982No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20720.13289923005677723No Hit
ATACTATAGCGACTCCGTGAGGGCCAGATTCACCATTTCCAGAGACAAGA20330.13039774840995563No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20200.12956392119434842No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20190.12949978063930173No Hit
TCTCTGGTCTCCAGGCTGAAGACGAGGCTGATTATTATTGTAGCTCATAT20170.12937149952920832No Hit
GTGTTGATACCAGGAGACATAACGATGAATATAAGTGACACCGCCGGTTGCAGTGCAGGAGATGGTGATGGACTG19270.12359884957500468No Hit
GGCTCAGCCTGCCTCCGTGTCTGGGTCGCCTGGGCAGTCCATCACCATCTCCTGCACTGCAACCGGCGGTGTC19240.12340642790986457No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG19160.1228933034694909No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCGTAGTCCCACTTCCAAAAACAAAAGGGCTGC19020.121995335698837No Hit
CTACTACTGTGCGACAAATAACTTGAACGGAGAACTTTCCTACTACTTTGACCACTGGGGCCAGGGAACCCTGGT18990.12180291403369689No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18800.12058424348780944No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG18380.11789034017584774No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18230.11692823185014713No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18030.115645420749213No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17990.11538885852902617No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG17260.11070659801061655No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17190.1102576141252896No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA17150.11000105190510276No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA16980.10891066246930875No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGATCTTCCTTGT16870.10820511636379497No Hit
GTATAACGTCGGTGACACATACTATAGCGACTCCGTGAGGGCCAGATTCACCATTTCCAGAGACAAGACCAAGA16860.10814097580874825No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCGCCTGGG16830.10794855414360813No Hit
GTTCTGTATAACGTCGGTGACACATACTATAGCGACTCCGTGAGGGCCAGATTCACCATTTCCAGAGACAAGACC16770.1075637108133279No Hit
CTTATATTCATCGTTATGTCTCCTGGTATCAACACCACCCAGGCAAAGTCCCCAAACTCCTTATTTTTGATGTC16670.10692230526286083No Hit
GTAGTAGAGAGCTGAGTCCTCGGCCCGCAGGTTGGCCATCTGAAGAAAAACCGTGTTCTTGGTCTTGTCTCTGG16550.10615261860230034No Hit
CAGTAGTAGAGAGCTGAGTCCTCGGCCCGCAGGTTGGCCATCTGAAGAAA16340.10480566694631949No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16300.10454910472613266No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG16290.10448496417108594No Hit
TCTCTGGATTGTCCGTCAGTGACTACTCGATAAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCT15930.1021759041894045No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15820.10147035808389072No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15790.1012779364187506No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG15650.1003799686480967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)