FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004718083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714372
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGGTCGGTCCCTCCGCCGAATACCAAAGTGTAGGCGTTTACATATGAGC73060.426161883185213No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC71950.41968720907714313No Hit
TCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT49870.2908936916841852No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45140.2633034137281757No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTACTTATAACTATGTCTCCTGGT43930.2562454356464058No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC41040.23938795080647607No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG40070.23372990226158616No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA39430.22999675682990622No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG38650.2254469858350463No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGGTCGGTCCCTC36910.21529749669266648No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34820.20310644364233665No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGTACTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC33440.1950568488052768No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG33190.19359858887102682No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG33080.19295695449995684No Hit
GATTATTACTGCTGCTCATATGTAAACGCCTACACTTTGGTATTCGGCGGAGGGACCGACCTGACCGTCCTGGGT32980.19237365052625685No Hit
GACCCAGGACGGTCAGGTCGGTCCCTCCGCCGAATACCAAAGTGTAGGCGTTTACATATGAGCAGCAGTAATAAT30290.17668277363372709No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT29880.17429122734155714No Hit
ATCCTGGGCTCAGTCCGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG29700.17324128018889717No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29520.17219133303623718No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG29510.17213300263886716No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC28920.16869150919403725No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC28580.16670827568345728No Hit
GTAATATACGGCCGTGTCCTCAGATGTCAGGCTGCTCATCTCCATGTAGACTGTGCTCGTGGACGTGTCCTTGGT28140.16414173819917732No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTGACCGTTTCCTGCAAGGCATCTGGGTACACCTTCAAC26900.15690876892529743No Hit
GGGCACAGGATCCTGGGCTCAGTCCGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC25970.15148404196988752No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25950.15136738117514753No Hit
ACCCAGGACGGTCAGGTCGGTCCCTCCGCCGAATACCAAAGTGTAGGCGTTTACATATGAGCAGCAGTAATAATC25910.15113405958566753No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25690.14985079084352754No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25440.14839253090927756No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25270.1474009141539876No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG24710.14413441190126763No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24700.14407608150389764No Hit
GTGTAGGCGTTTACATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC24440.14255949117227765No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA24240.1413928832248777No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTACTTATAACTATGTCTCCTGGTACCAACAGC24210.14121789203276766No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24170.1409845704432877No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCCGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGAC24060.1403429360722177No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG24040.1402262752774777No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT23930.1395846409064077No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATCATGACTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGG23620.13777639858793775No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA23530.13725142501160775No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG22940.1338099315667778No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC22610.13188502845356784No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22330.13025177732720788No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC22190.12943515176402787No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCCGCCCTGACTCAGC21750.1268686142797479No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC21560.12576033672971793No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG21350.12453539838494795No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20910.121968860900668No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC20640.12039394017167802No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCCGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT20170.11765241149528807No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19670.11473589162678813No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAAGTCATGATTTATGATGTCAATAAGCGGCCCTCAGGGGTCCCTG19480.11362761407675813No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19460.11351095328201814No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGACTCCAGG19090.11135272857932818No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19000.11082775500299819No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18910.1103027814266682No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18720.1091945038766382No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGT18610.10855286950556822No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCCGCCCTGACT18500.10791123513449823No Hit
CGCCTACACTTTGGTATTCGGCGGAGGGACCGACCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTC18270.10656963599498825No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17960.10476139367651828No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGG17960.10476139367651828No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGACTCC17860.10417808970281828No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC17860.10417808970281828No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA17580.10254483857645832No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAAGTCATGATTTATGATGTCA17560.10242817778171832No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17330.10108657864220834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)