FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004718084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714372
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGGTCGGTCCCTCCGCCGAATACCAAAGTGTAGGCGTTTACATATGAGC68210.3978716404607635No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64090.37383951674432386No Hit
TCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT47170.27514448439428546No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTACTTATAACTATGTCTCCTGGT44220.2579370171701358No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41480.24195448829075605No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA41440.24172116670127605No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG38080.22212215318495637No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG36010.21004776092936658No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35390.20643127629242664No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34270.19989827178698671No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGGTCGGTCCCTC33920.19785670787903675No Hit
GATTATTACTGCTGCTCATATGTAAACGCCTACACTTTGGTATTCGGCGGAGGGACCGACCTGACCGTCCTGGGT33070.19289862410258682No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGTACTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC32640.19039041701567688No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG31290.182515813370727No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT30540.1781410335679771No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG30450.1776160599916471No Hit
GTAATATACGGCCGTGTCCTCAGATGTCAGGCTGCTCATCTCCATGTAGACTGTGCTCGTGGACGTGTCCTTGGT30110.17563282648106712No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29330.1710830554862072No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC28350.1653666765439473No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28110.16396674700706731No Hit
GACCCAGGACGGTCAGGTCGGTCCCTCCGCCGAATACCAAAGTGTAGGCGTTTACATATGAGCAGCAGTAATAAT28050.1636167646228473No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG27240.1588920024358774No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC27090.1580170464753274No Hit
ATCCTGGGCTCAGTCCGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG26720.15585882177263746No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26580.15504219620945744No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTGACCGTTTCCTGCAAGGCATCTGGGTACACCTTCAAC26190.15276731071202748No Hit
GGGCACAGGATCCTGGGCTCAGTCCGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC25670.14973413004878755No Hit
GTGTAGGCGTTTACATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC25300.14757590534609757No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC25240.1472259229618776No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC25060.1461759758092176No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24980.14570933263025762No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG24860.14500936786181762No Hit
ACCCAGGACGGTCAGGTCGGTCCCTCCGCCGAATACCAAAGTGTAGGCGTTTACATATGAGCAGCAGTAATAATC23320.13602648666683775No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23210.13538485229576777No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTACTTATAACTATGTCTCCTGGTACCAACAGC22910.1336349403746678No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22650.13211835004304784No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCCGCCCTGACTCAGC22630.13200168924830782No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22600.13182669805619784No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCCGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGAC22350.13036843812194784No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATCATGACTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGG22230.12966847335350787No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC22110.1289685085850679No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG22080.12879351739295788No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA21900.1277435702402979No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT21490.12535202394812794No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21250.12395209441124797No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG20940.12214385209277799No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA20380.11887734984005806No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20240.11806072427687807No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19440.11339429248727814No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCCGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT19380.11304431010305815No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19230.11216935414250816No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC18810.1097194774529682No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18500.10791123513449823No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18430.10750292235290823No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAAGTCATGATTTATGATGTCAATAAGCGGCCCTCAGGGGTCCCTG18270.10656963599498825No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCCGCCCTGACT18100.10557801923969827No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18020.10511137606073828No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17810.10388643771596831No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGT17750.1035364553317483No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17550.10236984738434833No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGG17330.10108657864220834No Hit
CGCCTACACTTTGGTATTCGGCGGAGGGACCGACCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTC17190.10026995307902836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)