FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136557
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAAGACGGTCAGCTTGGTCCCTCCGCCGAATGTCAGAATGCTTCTGACCCACGCCTGACAG59690.5251826349228416No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG53360.4694881119028786No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG34400.3026684979283925No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31820.27996836058376307No Hit
CCCCAGGACAGACAGCCAGGATAACCTGCTCAGGAGATAAGTTGGAGAAG30170.26545083088661636No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAAGACGGTCAGCTTGGTCCCTC27730.24398248394053268No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27520.242134798342714No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC26040.2291130141295157No Hit
GTCTTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25320.2227780920798517No Hit
GTCTTGGTCATCTATCAAGATAATAAACGGCCCTCAGGGATCCCTGAGCG25270.2223381669375139No Hit
GGATAACCTGCTCAGGAGATAAGTTGGAGAAGAGATTTGCTTGGTGGTATCAGCAGAAGCCAGGCCAGTCCC24700.21732302031486325No Hit
CCCCTGTCTTGGTCATCTATCAAGATAATAAACGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTC23810.20949235278125075No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23780.20922839769584806No Hit
GACTATTACTGTCAGGCGTGGGTCAGAAGCATTCTGACATTCGGCGGAGGGACCAAGCTGACCGTCTTGGGTCAG23320.2051810863863405No Hit
GACCCAAGACGGTCAGCTTGGTCCCTCCGCCGAATGTCAGAATGCTTCTGACCCACGCCTGACAGTAATAGTCAG23210.2042132510731974No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22640.1991981044505467No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22520.198142284108936No Hit
CCTTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGGATAACCTGCTCAGGAGATAAGTTGGAGAAGAGATTTGCT21790.19171937703080444No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21110.18573639509501064No Hit
GGTCAGAAGCATTCTGACATTCGGCGGAGGGACCAAGCTGACCGTCTTGGGTCAGCCCAAGGCTGCCCCCTCGG20360.17913751795994393No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20300.17860960778913862No Hit
AGACAGGGGACTGGCCTGGCTTCTGCTGATACCACCAAGCAAATCTCTTCTCCAACTTATCTCCTGAGCAGGTTA20130.17711386230519016No Hit
CTATTACTGTCAGGCGTGGGTCAGAAGCATTCTGACATTCGGCGGAGGGACCAAGCTGACCGTCTTGGGTCAGCC20080.17667393716285237No Hit
GACCAAGACAGGGGACTGGCCTGGCTTCTGCTGATACCACCAAGCAAATC20050.1764099820774497No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19730.1735944611664879No Hit
GGATCCGTGGCCTCCGATGAGTTGACTCAGCCACCTTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGGATAAC19680.17315453602415012No Hit
GTAATAGTCAGCCTCATCGATAGCCTGGGTCCCGCTGATGGTCAGAGTGG19570.172186700711007No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19290.16972311991391545No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGTTTATTATCTTGATAGA19070.1677874492876292No Hit
GGAGATAAGTTGGAGAAGAGATTTGCTTGGTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTCTTGGTCATCTA19020.16734752414529144No Hit
GCACAGGATCCGTGGCCTCCGATGAGTTGACTCAGCCACCTTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGGA18850.16585177866134299No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18670.16426804814892698No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCGATGAGTTGAC18430.16215640746570564No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG18380.16171648232336786No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATCGATGAGGCTGACTATTACTGTCAGGCGTGGGTCAGAAGCA18320.16118857215256252No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCG18260.16066066198175719No Hit
AGCCAGGCCAGTCCCCTGTCTTGGTCATCTATCAAGATAATAAACGGCCCTCAGGGATCCCTGAGCGATTCTC18250.1605726769532896No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18020.15854902129853585No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17730.15599745547297672No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17560.1545017099890283No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17330.15247805433427447No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17310.15230208427733938No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17270.15195014416346914No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17200.15133424896419626No Hit
CTTCTGACCCACGCCTGACAGTAATAGTCAGCCTCATCGATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTG17040.14992648850871537No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA16970.14931059330944246No Hit
CAGTAATAGTCAGCCTCATCGATAGCCTGGGTCCCGCTGATGGTCAGAGT16810.14790283285396158No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16680.14675902748388336No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCGATGAG16040.14112798566195975No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15140.13320933309987973No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT15130.13312134807141215No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14980.13180157264439885No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14840.13056978224585306No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14820.13039381218891793No Hit
GAGTTGACTCAGCCACCTTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGGATAACCTGCTCAGGAGATAAGT14750.12977791698964505No Hit
GGCCAGTCCCCTGTCTTGGTCATCTATCAAGATAATAAACGGCCCTCAGG14590.12837015653416414No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAAGACGGTCAGCTTGGTCCCTCCGCCGAATGTCAGAA14530.1278422463633588No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG13470.11851583334579789No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG13220.11631620763410898No Hit
TCCTTGCTTACTGCACAGGATCCGTGGCCTCCGATGAGTTGACTCAGCCA13160.11578829746330364No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT13080.1150844172355632No Hit
GTCCCCTGTCTTGGTCATCTATCAAGATAATAAACGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAC13060.1149084471786281No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13050.11482046215016053No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12390.10901345027130183No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12240.1076936748442885No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12160.10698979461654803No Hit
GGACAGACAGCCAGGATAACCTGCTCAGGAGATAAGTTGGAGAAGAGATT12120.10663785450267782No Hit
GCTTGGTCCCTCCGCCGAATGTCAGAATGCTTCTGACCCACGCCTGACAGTAATAGTCAGCCTCATCGATAGC12110.10654986947421026No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA11960.10523009404719694No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11920.1048781539333267No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11850.10426225873405381No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG11700.10294248330704046No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT11640.10241457313623513No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAAGACGGTCAGCTTGGTCCCTCCGCCGAATGTCAGAATGCT11550.10162270788002714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)