FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136557
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAAGACGGTCAGCTTGGTCCCTCCGCCGAATGTCAG54970.48365370148615516No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT54160.4765269141802831No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33970.2988851417042876No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32890.28938275862979157No Hit
CCCCAGGACAGACAGCCAGGATAACCTGCTCAGGAGATAAGTTGGAGAAG27680.2435425587981949No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25700.2261215231616188No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAAGACGGTCAGCTTGGTCCCTC24970.21969861608348726No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA24380.2145074994039014No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG23910.2103722030659263No Hit
GGATAACCTGCTCAGGAGATAAGTTGGAGAAGAGATTTGCTTGGTGGTATCAGCAGAAGCCAGGCCAGTCCC23760.20905242763891296No Hit
CCCCTGTCTTGGTCATCTATCAAGATAATAAACGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG22960.20201362536150846No Hit
GACTATTACTGTCAGGCGTGGGTCAGAAGCATTCTGACATTCGGCGGAGGGACCAAGCTGACCGTCTTGGGTCA22940.2018376553045734No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22900.20148571519070313No Hit
GTCTTGGTCATCTATCAAGATAATAAACGGCCCTCAGGGATCCCTGAGCG22630.19911011942207912No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA22550.1984062391943387No Hit
GTCTTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG21670.19066355668919377No Hit
CCTTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGGATAACCTGCTCAGG21380.18811199086363464No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG20600.1812491586431653No Hit
GACCCAAGACGGTCAGCTTGGTCCCTCCGCCGAATGTCAGAATGCTTCTGACCCACGCCTGACAGTAATAGTCA20160.17737781739059283No Hit
GACCAAGACAGGGGACTGGCCTGGCTTCTGCTGATACCACCAAGCAAATCTCTTCTCCAACTTATCTCCTGAGCA19620.1726266258533448No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA19560.17209871568253945No Hit
AGACAGGGGACTGGCCTGGCTTCTGCTGATACCACCAAGCAAATCTCTTC18900.16629170380368077No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18880.16611573374674565No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG18490.16268431763651098No Hit
GTAATAGTCAGCCTCATCGATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGA18460.1624203625511083No Hit
GCACAGGATCCGTGGCCTCCGATGAGTTGACTCAGCCACCTTCAGTGTCCGTGTCCCCAGGACAGACAGCCAG18240.16048469192482206No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT18230.16039670689635452No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGTTTATTATCTTGATA18160.15978081169708164No Hit
CTATTACTGTCAGGCGTGGGTCAGAAGCATTCTGACATTCGGCGGAGGGACCAAGCTGACCGTCTTGGGTCAGCC18100.1592529015262763No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATCGATGAGGCTGACTAT18030.1586370063270034No Hit
GGATCCGTGGCCTCCGATGAGTTGACTCAGCCACCTTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGGATAAC17900.15749320095692518No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG17810.15670133570071715No Hit
CAGTAATAGTCAGCCTCATCGATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCA17730.15599745547297672No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT17640.15520559021676872No Hit
GGTCAGAAGCATTCTGACATTCGGCGGAGGGACCAAGCTGACCGTCTTGGGTCAGCCCAAGGCTGCCCCCTCGGT17600.15485365010289848No Hit
AGCCAGGCCAGTCCCCTGTCTTGGTCATCTATCAAGATAATAAACGGCCCTCAGGGATCCCTGAGCGATTCTCTG17590.15476566507443093No Hit
GGAGATAAGTTGGAGAAGAGATTTGCTTGGTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTCTTGGTCATCTAT17580.15467768004596338No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA17390.15300596450507983No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCGATGAGTTGACT17350.1526540243912096No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17260.1518621591350016No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG17110.15054238370798825No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17080.15027842862258559No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA16610.14614313228461046No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTG16400.14429544668679178No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTG16300.1434155964021162No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16280.1432396263451811No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15580.1370806743524522No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15430.13576089892543886No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA14980.13180157264439885No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTA14020.12335500991151346No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG13930.12256314465530546No Hit
GAGTTGACTCAGCCACCTTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGGATAACCTGCTCAGGAGATAAGTTG13800.12141933928522723No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGG13780.12124336922829211No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13770.12115538419982456No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC13760.121067399171357No Hit
CTTCTGACCCACGCCTGACAGTAATAGTCAGCCTCATCGATAGCCTGGGT13440.11825187826039521No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13170.1158762824917712No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13150.11570031243483608No Hit
GGCCAGTCCCCTGTCTTGGTCATCTATCAAGATAATAAACGGCCCTCAGG13030.11464449209322543No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAAGACGGTCAGC13000.11438053700782275No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAA12910.11358867175161474No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12790.11253285141000409No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12780.11244486638153652No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12640.11121307598299074No Hit
GTCCCCTGTCTTGGTCATCTATCAAGATAATAAACGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACT12240.1076936748442885No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCA12200.10734173473041828No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT12130.1067258395311454No Hit
GGACAGACAGCCAGGATAACCTGCTCAGGAGATAAGTTGGAGAAGAGATTTGCTTGGTGGTATCAGCAGAAGCCA12040.10593397427493738No Hit
GGCCTGGCTTCTGCTGATACCACCAAGCAAATCTCTTCTCCAACTTATCTCCTGAGCAGGTTATCCTGGCTGT11880.10452621381945648No Hit
TCCTTGCTTACTGCACAGGATCCGTGGCCTCCGATGAGTTGACTCAGCCA11820.10399830364865115No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT11720.1031184533639756No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT11650.10250255816470269No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAAGACGGTCAGCTTGGTCCCTCCGCCGAATGTCAGAATGCTT11630.10232658810776758No Hit
GCTTGGTCCCTCCGCCGAATGTCAGAATGCTTCTGACCCACGCCTGACAGTAATAGTCAGCCTCATCGATAGC11620.10223860307930002No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11420.1004789025099489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)