FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248775
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37050.29669075694180297No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT33260.26634101419391004No Hit
GTATCACTGTCAGCAGTTTGCTAACGCTCGGTACACTTTTGGCCAGGGGA23810.1906668535164461No Hit
CTCCTGCAGGGCCAGTCAGAATGTCACCACCAGGTACTTAGCCTGGTACC23470.18794418530159557No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT23020.18434065384076395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22470.17993633761085864No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21970.17593241376549018No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG21070.16872535084382695No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20240.1620788372605153No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC20160.16143820944525636No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG19950.1597565614302016No Hit
CAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC19810.15863546275349844No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA18930.15158855678564995No Hit
GTGTATCACTGTCAGCAGTTTGCTAACGCTCGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA18660.14942643790915097No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT18080.14478188624852356No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGTT17760.14221937498748774No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC17590.14085804088006246No Hit
AAAGTGTACCGAGCGTTAGCAAACTGCTGACAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTG16880.13517246901963925No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16860.1350123120658245No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCGAGCGTTAGCAAACTGCTGAC16510.13220956537406658No Hit
GTGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC16320.13068807431282659No Hit
GTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTACATCCAACAGGGCCG16160.12940681868230866No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG15920.1274849352365318No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG15790.12644391503673602No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15720.1258833656983844No Hit
GTCTCCGGGCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTCACCAC14910.11939700906888752No Hit
GGCCAAAAGTGTACCGAGCGTTAGCAAACTGCTGACAGTGATACACTGCA14350.11491261436207484No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG14170.11347120177774218No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13780.11034814117835479No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA13630.10914696402474426No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13450.1077055514404116No Hit
ACCTACTACAGCCCGTCCCTCAAGAGCCGAGTCACCCTATCCGTGGACACGTCCGAGAACCAGTTTTCCCTGA13050.10450241236411684No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCGAGCGTT12990.1040219415026726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)