FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412239
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATCACTGTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCT67600.478672519311533No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG58780.41621850125934773No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC56290.3985869247344111No Hit
GTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATGGCCAGGGAGGCAGAGGGGCCAGACTTGGAGCCAG56240.3982328770130268No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55150.3905146366868497No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49000.34696676695658457No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCATGCTGCACAGTGAT45340.321050473751256No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT44820.3173683774488596No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA42940.30405618312481103No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCATG42160.2985330386712164No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC42100.2981081814055553No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39160.2772901753881602No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG37920.2685097918978303No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT37740.267235220100847No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCC37360.2645444574183265No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC36770.26036669430599213No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC35180.24910797676597232No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT33430.23671630651752287No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG31990.2265197321416559No Hit
ATGCTGCACAGTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATG31950.22623649396454848No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTC31860.22559920806605682No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC31810.2252451603446725No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC30610.21674801503145008No Hit
GTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCA30340.214836157335975No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30140.21341996645043793No Hit
CTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCTCC29690.2102335369579795No Hit
TTCCTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA29310.20754277427545903No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29120.20619739293419884No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG27310.19338086542008825No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA27230.1928143890658734No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27120.192035484078828No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA26110.18488372010686577No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26040.18438805329692778No Hit
CCCCTGAGGGCCGCTGATCGTTCCTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATAA25900.18339671967705182No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATAAACAAAATTACTTCCGATGTTGGAGCTGCTTCCAGAAC25700.18198052879151475No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG25420.17999786155176284No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC24660.17461633618672193No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24550.17383743119967654No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC24360.17249204985841632No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC24090.17058019216294126No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC23620.1672521435819291No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22900.16215385639399565No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTTGTTTATTGGTACCAGCAGCTCCCAGGAACGGCCCC22890.16208304684971878No Hit
GCCCCCAGACTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT22770.16123333231839654No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCTCCCTGGCCATCAGTGGCCTCCGGT22480.15917985553436778No Hit
GCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG22410.1586841887244298No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT22350.15825933145876867No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCATGCTGCACAGTGATA22330.15811771237021496No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21980.15563937832052507No Hit
GTCTCTGGTGGCTCCATCATCAGTAGTACTTACTACTGGGGCTGGATCCG21680.15351509199221944No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21560.1526653774608972No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG21500.15224052019523607No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC21330.15103675794252955No Hit
GGCCATCAGTGGCCTCCGGTCCGACGATGAGGCTGATTATCACTGTGCAG20770.14707142346302574No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20520.14530118485610438No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG20470.14494713713472013No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20230.14324770807207562No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA20230.14324770807207562No Hit
ATCCCATGCTGCACAGTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATGGCCAGGGAGGCAGAGGGGCC20020.1417607076422617No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGG19790.14013208812389405No Hit
CCCAGGAACGGCCCCCAGACTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG19470.1378661827070347No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19210.1360251345558365No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT19190.1358835154672828No Hit
CTGATGGCCAGGGAGGCAGAGGGGCCAGACTTGGAGCCAGAGAATCGGTC18480.13085603782362618No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCT18240.12915660876098167No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTT18170.1286609419510437No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG17980.12731556060978347No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17940.12703232243267606No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAAC17870.12653665562273808No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17830.12625341744563068No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG17770.12582856017996955No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17730.12554532200286214No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTG17600.12462479792726303No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGC17530.12412913111732504No Hit
ACCTACTACAACCCGTCCCTCAACAGTCGAGTCACCATATCCGGAGACACGTCCAAGAACCAGTTCTCCCTGA17080.12094270162486662No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG17020.12051784435920548No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC16910.1197389393721601No Hit
ATAATACACAGCCGTGTCTGCGGCGGTAACAGATCTCAGCTTCAGGGAGA16880.11952651073932953No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16850.11931408210649898No Hit
GACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGATCGTT16460.11655250987970167No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC16390.11605684306976369No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC16220.11485308081705717No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15990.11322446129868953No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG15980.11315365175441268No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15760.11159584178032189No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15730.11138341314749131No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15690.11110017497038391No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15640.11074612724899964No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTC15550.11010884135050794No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT15220.10777212638937177No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGC15210.1077013168450949No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCC15190.10755969775654121No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC15130.1071348404908801No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG14940.10578945914961986No Hit
GTTCCTGGGAGCTGCTGGTACCAATAAACAAAATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTG14910.10557703051678931No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14730.10430245871980592No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14670.1038776014541448No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCATCAGTAGTACTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGA14660.10380679190986795No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT14620.10352355373276054No Hit
CATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCTC14550.10302788692282255No Hit
GTAGTAGGTGGTCCCACTAAAATACATATTCGCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC14420.10210736284722345No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC14350.10161169603728547No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTAACAGATCTCAGCTTCAGGGA14310.10132845786017805No Hit
TCAGTGGCCTCCGGTCCGACGATGAGGCTGATTATCACTGTGCAGCATGG14290.10118683877162434No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA14280.1011160292273475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)