FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412239
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATCACTGTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCT60850.4308760769246565No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC55270.39136435121817204No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG53720.38038887185525966No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53290.3773440614513549No Hit
GTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATGGCCAGGGAGGCAGAGGGGCCAGACTTGGAGCCAG49520.3506488632589809No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCATGCTGCACAGTGAT49520.3506488632589809No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCATG47460.33606209713794905No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA44770.31701432972747534No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43770.30993337529978987No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT40760.28861970247245683No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC36890.26121640883731434No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35990.2548435498523975No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCC35990.2548435498523975No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG34850.24677126180483616No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT34480.24415130866659254No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTC34240.24245187960394804No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT32890.23289259112657276No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC32320.2288564471027921No Hit
ATGCTGCACAGTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATG31710.224537064901904No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC31380.22220034994076782No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC30340.214836157335975No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC29690.2102335369579795No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29220.20690548837696737No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG28980.20520605931432287No Hit
CTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCTCC28250.2000369625821125No Hit
TTCCTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA28100.19897481941795972No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG27660.19585919946977812No Hit
GTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCA27370.19380572268574936No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC27240.19288519861015027No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATAAACAAAATTACTTCCGATGTTGGAGCTGCTTCCAGAAC26620.18849500686498533No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25530.18077676653880823No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24850.17596171752798218No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCATGCTGCACAGTGATA24430.1729877166683543No Hit
CCCCTGAGGGCCGCTGATCGTTCCTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATAA24430.1729877166683543No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA24200.17135909714998665No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24180.17121747806143295No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC23580.1669689054048217No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23360.16541109543073093No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA23230.16449057135513181No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG23160.16399490454519383No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC22530.15953390325575204No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC22110.15655990239612416No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22060.1562058546747399No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCTCCCTGGCCATCAGTGGCCTCCGGT21560.1526653774608972No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21350.15117837703108328No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTTGTTTATTGGTACCAGCAGCTCCCAGGAACGGCCCC20970.1484876143485628No Hit
GTCTCTGGTGGCTCCATCATCAGTAGTACTTACTACTGGGGCTGGATCCG20800.1472838520958563No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT20560.14558442303321179No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC20460.14487632759044325No Hit
GCCCCCAGACTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT20410.144522279869059No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG20350.14409742260339786No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC20300.1437433748820136No Hit
GGCCATCAGTGGCCTCCGGTCCGACGATGAGGCTGATTATCACTGTGCAG20070.14211475536364596No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19680.13935318313684866No Hit
GCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG19610.13885751632691068No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA19580.1386450876940801No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19100.13524622956879112No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18970.134325705493192No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18770.13290951460765493No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18730.13262627643054753No Hit
ATCCCATGCTGCACAGTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATGGCCAGGGAGGCAGAGGGGCC18670.1322014191648864No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGC18390.1302187519251345No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18320.1297230851151965No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCT17860.12646584607846123No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG17800.1260409888128001No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTG17520.12405832157304818No Hit
ACCTACTACAACCCGTCCCTCAACAGTCGAGTCACCATATCCGGAGACACGTCCAAGAACCAGTTCTCCCTGA17270.12228808296612685No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTT17240.12207565433329627No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC16900.11966812982788325No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16700.11825193894234615No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAAC16580.11740222441102392No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT16500.11683574805680909No Hit
ATAATACACAGCCGTGTCTGCGGCGGTAACAGATCTCAGCTTCAGGGAGA16270.11520712853844144No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16120.11414498537428863No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGG16110.11407417583001178No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG16030.11350769947579695No Hit
CCCAGGAACGGCCCCCAGACTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG16010.11336608038724323No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15910.1126579849444747No Hit
GTTCCTGGGAGCTGCTGGTACCAATAAACAAAATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTG15620.11060450816044592No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15530.10996722226195425No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG15430.10925912681918572No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15380.10890507909780142No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15350.10869265046497087No Hit
CTGATGGCCAGGGAGGCAGAGGGGCCAGACTTGGAGCCAGAGAATCGGTC15010.10628512595955784No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC14970.10600188778245043No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14900.10550622097251244No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGC14870.10529379233968188No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14740.10437326826408279No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14680.10394841099842167No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAAC14650.10373598236559109No Hit
GACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGATCGTT14590.10331112509992997No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC14450.10231979148005402No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT14410.10203655330294659No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCATCAGTAGTACTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGA14200.10054955287313266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)