FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004718101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822102
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67230.3689694649366501No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT61130.3354916464610653No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA53830.2954280276296278No Hit
GTCTCTGGTGGCTCCATCAGTAGTAACTACTGGAGCTGGATCCGGCAGCC50510.2772073133117685No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG45410.24921766179939434No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43250.23736322115885938No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40870.22430138378641812No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG40360.22150241863518066No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT38730.21255670648514738No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37580.20624531447745514No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA36970.20289753262989668No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG36940.20273288762100036No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAA36690.2013608458801977No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGC35780.19636661394367605No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA34040.18681720342768957No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT33760.1852805166779906No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTC33410.18335965824086686No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACAAGGAACTGTAACTCTGTTGAC33080.18154856314300735No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC32090.17611527784942885No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG31650.17370048438561617No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT31460.17265773266260614No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC31390.1722735609751814No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC31290.17172474427886034No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTAACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGG31140.17090151923437874No Hit
CCATCAGCAGTGTGCAAGCTGAAGATTTTGCAACTTACTACTGTCAACAG31130.17084663756474666No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG30670.16832208076166977No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG30480.16727932903865975No Hit
GAGTTACAGTTCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT30450.16711468402976343No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT30020.16475477223558285No Hit
GGCCAAAAGTGTACAAGGAACTGTAACTCTGTTGACAGTAGTAAGTTGCA28890.15855314356715486No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG28570.15679693013892745No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28530.15657740346039903No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT28230.15493095337143586No Hit
GCATAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT27040.1484000346852152No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26500.1454364245250815No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26360.144668081150232No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26180.14368021109685408No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA26050.1429667493916367No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24580.13489914395571706No Hit
GTAGTAAGTTGCAAAATCTTCAGCTTGCACACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC24370.13374662889344285No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACAAGGA23670.1299049120191954No Hit
GTGTATTACTGTGCGAGACGGTGGAGCAGTGGCTGGTACTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTC23230.12749011855538275No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23200.12732547354648643No Hit
ACTTACTACTGTCAACAGAGTTACAGTTCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA22880.125569260118259No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG22790.12507532509157007No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22700.12458139006488111No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA22670.1244167450559848No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC22660.12436186338635269No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTC22640.12425210004708848No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22360.1227154132973895No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG22240.12205683326180422No Hit
CACCATCAGCAGTGTGCAAGCTGAAGATTTTGCAACTTACTACTGTCAAC22210.12189218825290792No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC22200.1218373065832758No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC21960.12052014651210526No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC21750.11936763144983102No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGCTTGCACACTGCTGATGGTGAGAGTGAAATCTGTC21660.11887369642314205No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT21600.11854440640534941No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21580.11843464306608523No Hit
CCATATTAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGT21110.11585520459337623No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC21090.115745441254112No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC20530.11267206775471407No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20430.11212325105839299No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC20380.11184884271023246No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20060.11009262928200507No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19960.10954381258568402No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19870.10904987755899503No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAC19700.10811688917524924No Hit
GAACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGCTTGCACACTGCTGATGGTGAGAGTGAAAT19590.10751319080929608No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC19360.10625091240775762No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT19210.10542768736327604No Hit
GATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT18930.10389100061357706No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18850.10345194725652022No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18810.10323242057799178No Hit
GTATTACTGTGCGAGACGGTGGAGCAGTGGCTGGTACTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC18790.10312265723872757No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18660.1024091955335102No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18620.10218966885498176No Hit
CATCAGCAGTGTGCAAGCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCTTGTACACTTTTGG18430.10114691713197176No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC18270.10026881041785805No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18260.10021392874822596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)