FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137325
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAATGTTGCTGCCGGCATATGAGCAGCAGTAATAAT30540.2685248279955158No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT30390.26720594377156925No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGTAGTGACGTTGGTACCTATAACTCTGTCTCCTGGT29570.2599960433473282No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26390.23203569779966146No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC25560.22473787176049065No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGCTCTCCTGGACAGTCAGTCACCATCTCCTG24860.21858307871540678No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA24430.21480227727342666No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20440.17971995691644868No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGTCTCC19920.17514782494010067No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19910.1750598993251709No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC19790.17400479194601368No Hit
GATTATTACTGCTGCTCATATGCCGGCAGCAACATTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGT18780.16512430483810697No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18600.1635416437693711No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18570.16327786692458182No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18020.1584419581034445No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17390.15290264436286902No Hit
CTATAACTCTGTCTCCTGGTACCAACATCACCCCGGCAACGCCCCCCGAC16900.1485942892313103No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16300.14331875233552416No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16120.1417360912667883No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT16060.1412085375772097No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15960.14032928142791198No Hit
CTCCTGCACTGGAACCAGTAGTGACGTTGGTACCTATAACTCTGTCTCCTGGTACCAACATCACCCCGGCAACGC15800.13892247158903567No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15320.13470204207240674No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15170.1333831578484602No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG15010.1319763480095839No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC14580.12819554656760382No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14560.12801969533774427No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC14520.1276679928780252No Hit
TCTCTGGTCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT14230.12511815004506188No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13930.1224803815971688No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13880.12204075352251995No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13760.12098564614336273No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13720.12063394368364365No Hit
GAGTTATAGGTACCAACGTCACTACTGGTTCCAGTGCAGGAGATGGTGAC13690.12037016683885432No Hit
ATAATATATAGCCGTGTCTGCGGCGGTCACAGAATTCAGGGTCAGGTAGA12780.11236893588024532No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12720.11184138219066671No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12700.11166553096080717No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGCTCTCCTGGACAGTCAGTCAC12490.109819093047282No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12480.10973116743235223No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAATGTTG12240.10762095267403778No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12150.10682962213966983No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGACCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG12040.10586244037544237No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGTAGTGACGTTGGTACCTATAACTCTGTCTCCTGGTACCAACATC11950.10507110984107446No Hit
CCTGAGGGCCGCTCACTGACCTCAAAAATCATGAGTCGGGGGGCGTTGCCGGGGTGATGTTGGTACCAGGAGAC11940.10498318422614468No Hit
ATATATTATTGTGCGAGAAAAGTAGTGGGAGCCAACAATGCTTTTGACAT11860.10427977930670652No Hit
GGACTGGGGAAGCCCTCGGAGACCCTGTCGGAGTCGGGCCCAGGACTGGTGAAGCCCTCGGAGACC11830.10401600246191722No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11790.10366430000219815No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11750.10331259754247907No Hit
GTAGTGACGTTGGTACCTATAACTCTGTCTCCTGGTACCAACATCACCCC11650.10243334139318136No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG11510.1012023827841646No Hit
GTCCTGTCCCATGTGCAGCTACAGGAGTCGGGCACAGGACTGGGGAAGCCCTCGGAGACCCTGTCGGAGTCGGGC11470.10085068032444552No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG11400.10023520101993713No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCG11380.10005934979007759No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)