FastQCFastQC Report
Wed 8 May 2024
EGAF00004718106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137325
Total Bases83.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAATGTTGCTG37960.33376563427340467No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC32440.285230694832172No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC31150.27388829050623176No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGTAGTGACGTT29320.25779790297408395No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28690.25225858923350847No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28220.24812608533180927No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGCTCTC24780.21787967379596862No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA23030.20249269118325897No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT22900.20134965818917197No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20550.18068713868067615No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19000.16705866836656189No Hit
GATTATTACTGCTGCTCATATGCCGGCAGCAACATTTATGTCTTCGGAAC18740.1647726023783879No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG18730.1646846767634581No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG18540.1630140900797925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18150.1595849910975315No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17980.1580902556437254No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17670.15536456158090256No Hit
GGACTGGGGAAGCCCTCGGAGACCCTGTCGGAGTCGGGCCCAGGACTGGT17550.15430945420174533No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA16570.1456927439386279No Hit
CTCCTGCACTGGAACCAGTAGTGACGTTGGTACCTATAACTCTGTCTCCT16370.14393423164003252No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16200.14243949618622645No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16180.14226364495636692No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGT16080.14138438880706922No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15980.1405051326577715No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGACCAGAGACGGTCAGGGAGG15910.13988965335326314No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15850.1393620996636845No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15300.13452619084254722No Hit
CTATAACTCTGTCTCCTGGTACCAACATCACCCCGGCAACGCCCCCCGAC14920.13118501747521596No Hit
ATAATATATAGCCGTGTCTGCGGCGGTCACAGAATTCAGGGTCAGGTAGA14860.13065746378563733No Hit
GAGTTATAGGTACCAACGTCACTACTGGTTCCAGTGCAGGAGATGGTGAC14860.13065746378563733No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14840.1304816125557778No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14680.1290748027169015No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTC14650.12881102587211218No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14640.12872310025718242No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14550.1279317697228145No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14540.12784384410788474No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14380.1264370342690084No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA14300.12573362934957028No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG14070.12371134020618556No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13630.1198426131492757No Hit
TCTCTGGTCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT13540.11905128261490779No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT13410.11790824962082079No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA13170.11579803486250631No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG13160.11571010924757655No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12850.1129844151847537No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGG12810.11263271272503463No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12520.1100828698920713No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12470.10964324181742247No Hit
ATATATTATTGTGCGAGAAAAGTAGTGGGAGCCAACAATGCTTTTGACAT12300.10814850636361636No Hit
GTCCTGTCCCATGTGCAGCTACAGGAGTCGGGCACAGGACTGGGGAAGCC12260.10779680390389731No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12250.10770887828896752No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC12050.10595036599037214No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC11950.10507110984107446No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG11900.1046314817664256No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11870.1043677049216363No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11780.10357637438726838No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11770.1034884487723386No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG11650.10243334139318136No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11610.10208163893346228No Hit
CCTGAGGGCCGCTCACTGACCTCAAAAATCATGAGTCGGGGGGCGTTGCC11580.10181786208867298No Hit
TCCTTAGACACGTCCAAGAACCAGTTCTACCTGACCCTGAATTCTGTGAC11460.10076275470951575No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11410.10032312663486691No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11390.10014727540500737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)