FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011138
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT39200.3876819979073084No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37600.3718582428906836No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC27730.2742454541318792No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27250.2694983276268917No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG25230.24952083691840285No Hit
GTACTATTGTCAACAGACTGACAGTTTCCCTCTCACTTTCGGCGGCGGGA24450.2418067563477982No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23910.23646623902968733No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22100.2185656161671305No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21330.2109504340653798No Hit
ATTGTCAACAGACTGACAGTTTCCCTCTCACTTTCGGCGGCGGGACCAAGGTTGAGATCAAGCGAACTGTGGCTG21300.21065373865881806No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA20760.2053132213407072No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT20490.20264296268165177No Hit
GAATACAGGAGACTCGCCATGGAGTTTGGGCTGAGCTGGGTCTTCCTTGT20040.19819253158322603No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC20030.19809363311437214No Hit
GTATTAAGAGTTGGTTAACCTGGTATCAGCAGAGACCGGGGAAAGCCCCTCAGCTCCTGATCTTCGCTGCATACA19880.19661015608156354No Hit
GGGTATTAAGAGTTGGTTAACCTGGTATCAGCAGAGACCGGGGAAAGCCCCTCAGCTCCTGATCTTCGCTGCAT19000.18790709082241988No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18690.18484123828794882No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18000.17801724393702936No Hit
GTTGACAATAGTACGTTGCAAAATCCTCAGGCTGAAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAAATCC17940.17742385312390593No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC17380.17188553886808725No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17140.1695119756155935No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG16910.1672373108319537No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA16500.16318247360894358No Hit
CAATAGTACGTTGCAAAATCCTCAGGCTGAAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAAATCCACTGCCG16420.16239128585811236No Hit
ATTGGAGACAGAGTCACCATCTCTTGTCGGGCGAGTCAGGGTATTAAGAGTTGGTTAACCTGGTATCAGCAG16370.1618967935138428No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT16340.16160009810728113No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16120.15942433179249518No Hit
GTAATAGACGGCCGTGTCGTCGGTTTTCAGGCTGTTCATTTGGAGATACGCCGTGTTCTTTGAATCATTTCTGG15880.15705076854000147No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14980.14814990634314998No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14500.14340277983816255No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC14480.14320498290045475No Hit
GTCTAGTACAGTAATAGACGGCCGTGTCGTCGGTTTTCAGGCTGTTCATTTGGAGATACGCCGTGTTCTTTGAAT14430.1427104905561852No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14370.14211709974306178No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13820.136677683956097No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG13740.13588649620526577No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13670.1351942069232884No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13640.13489751151672671No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC13550.13400742529704157No Hit
GATTTCACTCTCACCATCAACAGCCTTCAGCCTGAGGATTTTGCAACGTACTATTGTCAACAGACTGACAGTTTC13480.13331513601506423No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13450.13301844060850251No Hit
GCATACAATCTGCATACTGGGGTTCCATCAAGGTTCAGCGGCAGTGGATTTGGGACAGATTTCACTCTCACCATC13360.13212835438881737No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13160.13015038501173926No Hit
GTACTAGACATGTGGGTGACTACAGTGACCACTATCCATTTGATTACTGGGGCCAGGGAACCCAGGTCACCGTCT13100.12955699419861583No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13020.12876580644778457No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12930.12787572022809943No Hit
CACCATCAACAGCCTTCAGCCTGAGGATTTTGCAACGTACTATTGTCAACAGACTGACAGTTTCCCTCTCACTTT12790.12649114166414477No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12630.12490876616248227No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC12630.12490876616248227No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12600.12461207075592055No Hit
CTGTAGTCACCCACATGTCTAGTACAGTAATAGACGGCCGTGTCGTCGGT12490.12352418759852761No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12470.12332639066081978No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12440.12302969525425807No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC12280.12144731975259558No Hit
CTCTTAATACCCTGACTCGCCCGACAAGAGATGGTGACTCTGTCTCCAATAGAAGCAGACACGGAAGATGG12070.1193704519066636No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11960.11828256874927062No Hit
CTTAATACCCTGACTCGCCCGACAAGAGATGGTGACTCTGTCTCCAATAG11900.11768917793614718No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11870.11739248252958547No Hit
CTCCTGTGCAATCTCTGAGTTCAACTTCGATGAGTCTCCTATTCATTGGGTCCGCCAGGCTTCCGGGAAAGGG11710.11581010702792298No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC11610.11482112233938394No Hit
GTGCAGCCACAGTTCGCTTGATCTCAACCTTGGTCCCGCCGCCGAAAGTGAGAGGGAAACTGTCAGTCTGTTGAC11500.11373323918199099No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC11380.11254645755574412No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11310.11185416827376679No Hit
GTCTCCATCTTCCGTGTCTGCTTCTATTGGAGACAGAGTCACCATCTCTT11260.11135967592949725No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11020.10898611267700353No Hit
GTCTATTACTGTACTAGACATGTGGGTGACTACAGTGACCACTATCCATT10940.10819492492617228No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10670.10552466626711685No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10650.10532686932940903No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC10450.10334889995233093No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC10440.10325000148347703No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT10400.10285440760806142No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC10350.10235991526379189No Hit
GGTTGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGG10340.10226101679493799No Hit
GGTATCAGCAGAGACCGGGGAAAGCCCCTCAGCTCCTGATCTTCGCTGCATACAATCTGCATACTGGGGTTCCAT10220.10107423516869112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)