FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011138
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37580.37166044595297576No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT32340.3198376482735294No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC24770.24497150735112316No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA24760.24487260888226928No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24180.23913649768874276No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG22270.22024689013764692No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21710.2147085758818282No Hit
GTACTATTGTCAACAGACTGACAGTTTCCCTCTCACTTTCGGCGGCGGGA20240.20017050096030414No Hit
GTTGACAATAGTACGTTGCAAAATCCTCAGGCTGAAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAAATCC20190.1996760086160346No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT19730.19512667904875497No Hit
ATTGTCAACAGACTGACAGTTTCCCTCTCACTTTCGGCGGCGGGACCAAGGTTGAGATCAAGCGAACTGTGGCTG19310.19097294335689094No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18850.1864236137896113No Hit
GAATACAGGAGACTCGCCATGGAGTTTGGGCTGAGCTGGGTCTTCCTTGT18090.1789073301567145No Hit
GTGCAGCCACAGTTCGCTTGATCTCAACCTTGGTCCCGCCGCCGAAAGTGAGAGGGAAACTGTCAGTCTGTTGAC17990.17791834546817548No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17920.17722605618619813No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC17860.1766326653730747No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC16700.1651604429860217No Hit
CAATAGTACGTTGCAAAATCCTCAGGCTGAAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAAATCCACTGCCG16500.16318247360894358No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16350.161698996576135No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG16320.1614023011695733No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15640.15467720528750775No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15600.15428161141209212No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG15590.1541827129432382No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15310.15141355581532887No Hit
GTATTAAGAGTTGGTTAACCTGGTATCAGCAGAGACCGGGGAAAGCCCCTCAGCTCCTGATCTTCGCTGCATACA14850.14686422624804923No Hit
GGGTATTAAGAGTTGGTTAACCTGGTATCAGCAGAGACCGGGGAAAGCCCCTCAGCTCCTGATCTTCGCTGCAT14670.14508405380867895No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC14550.14389727218243206No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14080.13924904414629852No Hit
GTAATAGACGGCCGTGTCGTCGGTTTTCAGGCTGTTCATTTGGAGATACGCCGTGTTCTTTGAATCATTTCTGG13990.13835895792661337No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13890.13736997323807432No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT13870.13717217630036652No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13730.13578759773641183No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT13630.1347986130478728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA13480.13331513601506423No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC13110.1296558926674697No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12960.12817241563466114No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC12940.12797461869695334No Hit
GTACTAGACATGTGGGTGACTACAGTGACCACTATCCATTTGATTACTGGGGCCAGGGAACCCAGGTCACCGTCT12850.1270845324772682No Hit
GTCTAGTACAGTAATAGACGGCCGTGTCGTCGGTTTTCAGGCTGTTCATTTGGAGATACGCCGTGTTCTTTGAAT12700.1256010554444596No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12700.1256010554444596No Hit
CTCCTGTGCAATCTCTGAGTTCAACTTCGATGAGTCTCCTATTCATTGGGTCCGCCAGGCTTCCGGGAAAGGG12630.12490876616248227No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCAACCTTGGTCCCGCCGCCGAAAGTGAGAGGGAA12530.12391978147394322No Hit
ATTGGAGACAGAGTCACCATCTCTTGTCGGGCGAGTCAGGGTATTAAGAGTTGGTTAACCTGGTATCAGCAG12350.12213960903457292No Hit
CTCTTAATACCCTGACTCGCCCGACAAGAGATGGTGACTCTGTCTCCAATAGAAGCAGACACGGAAGATGG12310.12174401515915731No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12230.12095282740832607No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12110.11976604578207921No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC11930.11798587334270891No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11890.11759027946729328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11820.11689799018531595No Hit
GATTTCACTCTCACCATCAACAGCCTTCAGCCTGAGGATTTTGCAACGTACTATTGTCAACAGACTGACAGTTTC11820.11689799018531595No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11770.11640349784104642No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC11730.11600790396563081No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAAATCCACTGCCGCTGAACCTTG11710.11581010702792298No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11670.11541451315250736No Hit
ATCTCAACCTTGGTCCCGCCGCCGAAAGTGAGAGGGAAACTGTCAGTCTGTTGACAATAGTACGTTGCAAAATC11570.11442552846396832No Hit
CTTAATACCCTGACTCGCCCGACAAGAGATGGTGACTCTGTCTCCAATAG11340.1121508636803285No Hit
GAGTGAAATCTGTCCCAAATCCACTGCCGCTGAACCTTGATGGAACCCCAGTATGCAGATTGTATGCAGCGA11130.11007399583439648No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG10920.10799712798846449No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC10910.10789822951961059No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT10680.10562356473597075No Hit
GGTTGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGG10640.10522797086055513No Hit
GCATACAATCTGCATACTGGGGTTCCATCAAGGTTCAGCGGCAGTGGATTTGGGACAGATTTCACTCTCACCATC10630.10512907239170123No Hit
ACCTTGGTCCCGCCGCCGAAAGTGAGAGGGAAACTGTCAGTCTGTTGACAATAGTACGTTGCAAAATCCTCAGG10580.1046345800474317No Hit
GGCTGAAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAAATCCACTGCC10520.10404118923430826No Hit
CACCATCAACAGCCTTCAGCCTGAGGATTTTGCAACGTACTATTGTCAACAGACTGACAGTTTCCCTCTCACTTT10240.10127203210639893No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10170.1005797428244216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)