FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17493667
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGC18850.016.87107370
GTCGCTA17000.013.90803427
GTCCGAT17900.013.2096171
CTACCGG18700.012.94855269
TCCGATT23500.012.5734662
CGCTATG19150.012.52805329
TCGCTAT19800.011.94134428
CGCGTAT2953.0965653E-511.7872451
TCGCATT26050.011.3426672
GACCGTT24050.011.27678526
CCGATTG15600.011.1416793
CTCGCAT31850.011.1358941
CGCGTTA21150.011.01488934
AGCGACG23150.010.96224321
CCCGTCT54850.010.9040081
ACGTACG9450.010.67464253
ACTATCG19400.010.58169958
CCCGTAT21450.010.5370831
CGTATCG20750.010.3867293
TCGTATG9800.010.29184445
Produced by FastQC (version 0.11.5)