FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17493667
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG278530.15921761858162728No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN253470.1448924345021544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC28250.018.2762281
TAACACG26800.017.4535474
TCGCACG29150.015.47574827
CGCACGA32700.014.11357628
AACACGT35850.013.5382745
CGCGAGT34300.013.43731413
GCGAACG10400.013.4221293
TGCGCGA37000.012.93195611
GCACGAA36750.012.6525929
ACGTGCG37450.012.5903018
GCGCAAT37500.012.39939546
TAGGTCG7350.011.3951143
CGAGTCG40500.011.200644515
CGCTCGC63450.011.08594270
CGGCGAA16200.010.7670891
GCGAGTC43700.010.54566114
ACACGTG47150.010.4430286
ACGAAAG47500.010.299831
TACGCAC12400.010.139969
TCTAACA52600.010.0823362
Produced by FastQC (version 0.11.5)