FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17830148
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGC17750.021.8737570
GTCCGAT18400.015.6857921
CTACCGG18950.015.39303469
CCGATTG15100.014.0454523
GTCGCAT9950.012.9307831
CGATTGG15900.012.6827224
TCCGATT25700.012.1746272
GCTACCG23300.011.92196868
CGCTACC22350.011.90190767
CCGGTAT37250.011.2021441
GTTCGAT24650.011.1443911
GCCGCTA24450.011.11053365
GTCGCTA16900.011.11000427
GTATCGA14150.011.057014
GCGAGAC37250.010.97164470
CCCGTCT54600.010.8905461
CGATTAG19850.010.684424
CGCTACG16350.010.64078959
ACGACCG16400.010.60055336
GACCGTT23650.010.43841226
Produced by FastQC (version 0.11.5)