FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17830148
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG283170.1588152829690477No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN206310.11570851795509494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC26950.016.4282051
TAACACG26150.016.4062944
TCGCACG27950.014.65281127
AACACGT32800.013.2948035
CGCGAGT32350.013.16079813
TAGGTCG9500.012.8484013
ACGTGCG34900.012.6004838
CGCACGA34050.012.33298728
GCACGAA35250.011.81405429
GCGAACG10800.011.6247433
TGCGCGA37300.011.60239311
GCGCAAT37100.011.31937546
ACCGGTA13450.011.09557545
TACCGGT13300.010.95970444
CGAGTCG38850.010.858417515
CGGCGAA15850.010.7773511
GCGAGTC39950.010.74292214
CGCTCGC63550.010.52539870
TTGTCGT36700.010.47628270
TTAGCGG11350.09.8400819
Produced by FastQC (version 0.11.5)