FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17655462
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257690.14595483256116434No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN216300.12251166239659998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC26850.013.5060981
TAGGTCG7500.013.4873013
TACCGGT11700.013.05274144
TAACACG26300.012.60296254
ACCGGTA12350.011.52286345
TCGCACG28950.011.26974727
AACACGT35750.010.9331975
CGCGAGT31100.010.77095213
CTTGACG17950.010.4898711
ACGTGCG33300.010.4816398
CGCACGA32650.010.31159628
GCGCAAT34700.010.10288146
CGACCTA12100.010.0922384
GCGAACG11450.010.0530253
CGGTATT16300.010.00863247
TGCGCGA35750.09.86066811
GCACGAA35050.09.80360429
TACGCAC13550.09.7885079
TTGTCGT36350.09.37483670
CGAGTCG40300.08.65169315
Produced by FastQC (version 0.11.5)