FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15901104
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN232010.1459081080156447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGG36500.017.06003470
GCGCTAA23250.016.26535642
CGACCGT16950.015.0217711
TCCGCAT53350.014.73813770
CGCTAAG26550.014.37498443
CATATAG43800.014.11112512
TTGTCGT27000.014.07211770
CGCTAGG9450.012.88359418
CCGTTGA21500.012.1755784
GCGATAG29600.012.11049566
ATAGGCG30550.011.82564362
CGATAGA30600.011.77281567
TACCGGT11250.011.72023544
GCATTCG15900.011.4118833
CCGTCAT36950.011.3275181
CATTCGG15750.011.3020294
ACCGTTG23000.011.2267753
CGCAAAA52400.011.1826811
TACTCTA37200.011.17137059
CCGTATG19800.010.93504711
Produced by FastQC (version 0.11.5)