FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16239253
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA27150.027.1430824
CCCGTAT31000.026.915031
CGTATCG28300.026.7770793
TATCGAA33300.023.1742615
CCGTATC35000.023.039142
ATCGAAG50400.017.1048646
ATCGCGT44450.016.38848367
CGTGTTA48550.014.8371159
CGCGTCA55100.014.61786469
GTCCGAT13350.014.5831751
TCGCGTC50900.014.56435368
TACATCG51400.014.36529764
ACATCGC52800.013.92393565
TATGCCG7250.013.43075648
CATCGCG55950.013.21603666
GCGTCAT80050.013.1367170
TCGTGTT58300.012.59360258
CTCGTTT26400.012.3785341
TCGAAGG73750.011.9720597
ACGGTTA16100.011.87376712
Produced by FastQC (version 0.11.5)