FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16062934
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN196720.1224682863043576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAA24550.017.66939542
GAGTAGG39750.017.5429470
CGCTAAG28200.015.87562743
CATATAG45750.013.80679312
CCGTCAT36250.013.4667571
TCCGCAT55300.013.27868970
ATAGGCG31150.013.27518762
GCGATAG30100.013.06984866
CGACCGT16300.013.0492391
TTGTCGT25150.012.81319570
CGATAGA31500.012.55207367
TAGGTCG7700.012.2306093
GTCGTAA8350.012.1194865
TACCGGT13750.012.1153444
AGCGCTA36300.012.04522441
TACTCTA36900.011.7288449
ACCGGTA13950.011.44450445
CCGTATG19600.011.39617711
CGAACGA28200.011.33293959
TAGGCGA38500.011.284574563
Produced by FastQC (version 0.11.5)