FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14009706
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN205360.14658408963043193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG11650.020.6828386
GTCGTAA12350.019.7907985
GCGAACG8650.016.9444583
CGACCTA9550.016.8141164
TACGCAC10200.016.0940769
GCGGTCG14900.015.9219112
CTTAGCG9800.015.6817658
GGTCGTA16750.014.5882394
GCGTTCG12950.014.2805315
TTGTCGT34750.013.88532370
GCGCTAA18450.013.16178542
GAGTAGG31900.013.13124370
TTAGCGG12450.012.9049649
CGTAAGG23450.012.8087497
CGCGGTC19350.012.7992911
CGTTCGC14750.012.76152216
TAATACG10900.012.73673324
ACACGCG18700.012.64474269
TTCGCAC15700.012.63078518
TAGGTCG9600.012.369697
Produced by FastQC (version 0.11.5)