FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14257979
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN163430.11462353816063273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG10100.020.3802976
CTTAGCG8250.017.7687918
GTCGTAA13050.016.8419155
CGACCTA10800.015.82593254
GCGGTCG13300.015.731582
GTCTAAC15300.015.4955091
TTGTCGT38550.014.95110670
TCGCACG15800.014.93704227
TACGCAC12550.014.7399059
GCGTTCG13350.014.62554315
GGTCGTA16350.013.8673214
GCGAACG8850.013.7933943
TTCGCAC15500.013.68723618
GCATTCG12450.013.4467773
TGCGCGA17500.013.36252411
CGTTCGC14200.013.25132116
CGGTAGG9600.012.66085860
CGCACGA17950.012.56742928
CGCGGTC19700.012.565521
CGCGCTA13000.012.54832246
Produced by FastQC (version 0.11.5)