FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14120804
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174280.12342073440010923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG11350.023.9822336
GTCGTAA12650.021.5163485
GGTCGTA16550.018.3393964
GCGGTCG14500.018.0372812
CGACCTA9700.017.2636744
TAGGTCG8500.016.8243083
GCGAACG8000.016.5678423
TTGTCGT35800.016.2678970
CTTAGCG9150.015.6387948
CGCGGTC19550.015.5170251
CGTAAGG23750.014.4011877
CGTTCGC12450.014.26835316
GCGTTCG12100.014.11948315
GAGTAGG29450.014.02267670
TACGCAC12200.013.7315739
CGCTTGT22150.013.39209312
TTAGCGG12800.013.3605719
GCGCTAA19550.013.31363542
CGGTCGT20500.013.1011433
TCCGCAT42000.012.73191470
Produced by FastQC (version 0.11.5)