FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14880096
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN195310.13125587361801969No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181600.12204222338350505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT34700.022.5018749
GTCTAAC25600.020.9702431
GTACGCA25350.020.9478350
CGTCGAT7950.020.19708812
TCTCGAT38550.020.16213248
ATTCCGT38100.019.86601853
ACGCACG26700.019.76200952
TTCTCGA39450.019.6117647
ACGGACA40250.019.44198418
AGTACGC28100.019.26565749
TACGCAC27750.018.88751
CGCGAGT26800.018.75499313
TGCGCGA27550.018.24553511
ACACGGA44300.018.09610416
ACGTGCG27700.017.8947548
CGGACAG44400.017.61923819
CGGACAC46250.017.50916713
TCGATTC45800.017.35383850
CCGGTAC30800.017.2755760
CGCACGA29000.016.86220728
Produced by FastQC (version 0.11.5)