FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14579632
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9850.030.3453246
GTATCGA22800.029.2408874
CCCGTAT28000.028.5307461
TCGTATG10850.027.86272443
TATCGAA26700.025.750055
CGTATGC13750.023.50420844
GATCGGA15250.023.4590641
CGTATCG38800.021.120443
CCGTATC44650.020.1417872
ATCGAAG55800.019.664376
CTCGTAT15700.018.5901342
ATCGCGC38350.017.5851367
CATCGCG41750.017.33705366
TACATCG38800.017.08866764
ACACGTC22950.016.49224913
CGTGTTA40800.016.13581359
ACATCGC45350.015.86046165
CGCGCCA48000.015.580169
GTCGCTA21850.015.25946227
TCGAAGG70900.015.0357037
Produced by FastQC (version 0.11.5)