FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14579632
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN214410.1470613250046366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA24050.023.089550
TACGCAC25450.022.09589451
CTCGATT37350.021.6483949
ACGCACG25800.021.52987152
TTCTCGA38450.020.93556447
GTCTAAC23350.020.4537431
CGTCGAT7050.020.29612212
ATTCCGT39550.020.2805653
AGTACGC27900.020.27419749
CCGGTAC28950.019.9428160
TCTCGAT41350.019.7207248
ACGGACA41850.019.69050818
ACGTGCG26050.018.6237018
TGCGCGA25000.018.42804511
CGGACAG46200.018.13163219
CGCGAGT25200.018.00037213
CGCACGA26200.017.60492528
CGAGTCG25750.017.60383615
CGGACAC47200.017.5204413
ACACGGA46750.017.3616416
Produced by FastQC (version 0.11.5)