FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14876823
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10050.025.58908543
GTATCGA23100.025.4091844
GATCGGA15500.025.3242861
CCCGTAT27150.025.205011
TATGCCG10850.024.98261546
TATCGAA25700.023.3793225
GTCGCTA24350.020.3972727
CGTATGC13700.020.04098144
CTCGTAT13650.019.6025342
ATCGAAG58650.019.6011646
CGTATCG36550.019.2898583
CGCTATG26000.019.2363529
ACGTCGC26950.018.42870125
GCGACGT27000.018.2650822
CCGTATC42900.017.6474362
ACACGTC23900.017.4371713
CGACGTC29150.017.15642423
TACCGGC23950.017.09504170
TCGCTAT29650.016.9854428
CCGACAT34500.016.814651
Produced by FastQC (version 0.11.5)