FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14720655
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10800.032.50258346
TCGTATG11450.027.31221643
GTATCGA23600.026.633414
CGTATGC13550.025.90171644
GATCGGA17200.025.045291
CCCGTAT27200.024.7779641
TATCGAA27400.023.9536345
CTCGTAT15650.019.9814742
CGTATCG39250.019.8185333
ATCGAAG58700.019.818396
GTCGCTA24450.018.18328927
CGCTATG25300.017.84701729
CGCGTAT2156.093069E-817.77679442
CCGTATC45150.017.7671342
ACACGTC24650.017.32805613
GCGACGT26100.017.16492322
ACGTCGC26500.017.03705625
ATCGCGC38400.016.46414267
CATCGCG43200.016.4330966
TCGCTAT28450.016.23729328
Produced by FastQC (version 0.11.5)