FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12537942
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13350.041.147946
TCGTATG14200.038.6889943
GATCGGA16750.033.198571
CGTATGC17950.031.18784144
CTCGTAT21800.024.08323742
CGTCTGA23800.023.94406716
ACACGTC24600.023.44752913
ATCGGAA25100.022.4258962
GTATGCC25950.022.10739345
ACGTCTG27550.020.43263815
GTCCGAT12200.017.662251
CACGTCT34500.016.92047714
TCGGAAG33550.016.8830283
CGTACGA5900.016.5097254
GCCGTCT34300.016.42290549
ATGCCGT39800.015.28819947
CACACGT38550.015.23297412
CGGAAGA39700.015.1429034
CGATTGG11550.015.0432464
CCGATTG11350.015.0022183
Produced by FastQC (version 0.11.5)