FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12716372
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG9350.022.31665445
TACCGGC14550.021.63778570
TATGCCG10500.020.53793548
GATCGGA11000.019.2799221
CGTATGC11450.019.13580346
GTCCGAT14100.017.9999751
CGATTGG9800.016.6681044
CTACCGG14500.016.16005369
CCGATTG11150.015.5853433
CCCGTAT14100.015.287651
CGCTATG16050.015.16142129
GTCGCTA16450.015.00407927
CGCGTTA20300.014.89859334
TCCGATT18900.014.8950772
TATTGCG19450.014.4739367
ATCGGAA15400.013.9923462
AGGGGGG78950.013.9596741
CGGGAAT34550.013.7860751
TCGGTCG22850.013.69219829
CGCTACC16250.013.37806167
Produced by FastQC (version 0.11.5)