FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12716372
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG388910.30583408538221435No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN187340.1473218933827982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC18550.019.1731011
ACCGGTA10400.018.01095845
TACCGGT10400.018.01024844
CGGTATT12150.016.27449447
TTGTCGT26500.015.767693570
TAACACG20650.015.546574
TCGCACG21400.015.23638327
TGCGCGA23700.014.28929411
CGCGAGT23150.014.0220813
CCGTTGT14150.013.97068737
CGCACGA24800.013.42688828
AACACGT25500.013.0038185
ACGTGCG25500.012.8703578
GTGTCGC16350.012.5978812
TAGGTCG5307.2759576E-1212.5158877
CCCGTTG16300.012.3402636
GCGCAAT27050.012.18302146
GCACGAA26900.012.12075529
AGCCGTA17000.011.85948563
GCGAGTC28850.011.61330814
Produced by FastQC (version 0.11.5)