FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13002128
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10150.025.694545
TATGCCG10450.023.29539948
GATCGGA10700.022.7435871
TACCGGC15050.018.5662170
CGTATGC13700.018.52858446
CCCGTAT13950.017.1956881
CGCGTAT2251.0173244E-716.9963171
GTCCGAT12550.016.0667821
ATCGGAA15300.015.9019752
AGGGGGG79050.015.0847081
CGATTGG9400.014.7917664
CTACCGG15550.014.08117469
TCCGATT20150.013.9718672
CGCTATG14600.013.80812329
TATTGCG16900.013.780697
CCGATTG10950.013.6503543
GTATCGA10700.013.6443684
GTCGCTA15100.013.581140527
CGGGAAT35050.013.3903051
ACTATCG18750.013.1835458
Produced by FastQC (version 0.11.5)