FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12828321
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG8650.025.33073445
TATGCCG8900.025.01232148
GATCGGA10250.022.3874441
TACCGGC13950.018.90923770
CGTATGC12800.016.84783246
CGCGTTA19550.016.71519334
CCCGTAT13050.016.2518861
CGATTGG10100.015.4849454
GTCGCTA16850.015.47336827
ATCGGAA14600.015.47312
CGCTATG17450.015.33934929
TTACCGC23050.015.08436738
CCGATTG11500.014.8086953
CTACCGG14000.014.8012769
GTATCGA9000.014.6743754
TCGGTCG22750.014.36342729
GTCCGAT15100.014.2757591
CGACGTC19400.014.15455923
GCGACGT20200.014.1099922
ACGTCGC19050.013.86733525
Produced by FastQC (version 0.11.5)