FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12828321
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG351740.27419020774425584No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN160310.12496569114539618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC19950.021.3124071
TAACACG22250.018.4946254
TCGCACG25550.016.0223627
AACACGT27900.015.7534585
TGCGCGA26800.015.75161311
CGCGAGT25750.015.71091913
ACGTGCG27250.015.6205678
CGCACGA26500.015.31729828
TACGCAC7950.014.4827249
CGCTCGC46000.013.78116870
GCGCAAT30450.013.56238246
GCGAGTC31200.013.52289714
TTGTCGT27900.013.44363770
GCACGAA31100.013.38611529
CGAGTCG31200.013.29149315
ACACGTG33000.013.21437556
ACCGGTA9550.012.71745445
CGACCTA8250.012.6812154
TCTAACA37300.012.6152262
TAGGTCG5409.094947E-1212.2668293
Produced by FastQC (version 0.11.5)