FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16944100
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA17250.024.7739324
CCCGTAT21500.024.4088971
CGTATCG20450.022.9362263
TATCGAA21550.020.153215
CCGTATC30050.018.0368352
ATCGAAG40300.016.0359426
ATCGCGC27650.014.95403867
TACCGGC14550.014.66618870
CATCGCG29550.014.643366
TACATCG28750.014.17897864
CCGGTAT25300.013.5995441
CGTGTTA30350.013.18834459
ACATCGC33650.013.15533665
CTCGCAT29800.012.9454271
TCGCATT22850.012.7724232
CCCGTCT61050.012.637961
CGCTAAT6150.012.4324651
CTCGTTT27150.012.4168531
TCTACTA77050.011.5890997
CTCCGAT35150.011.3705681
Produced by FastQC (version 0.11.5)