FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16944100
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN251040.14815776582999393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG19850.013.63373427
CGCGCTA11450.013.0304446
CGCACGA21300.012.86793728
GTCTAAC26600.011.8009271
TGCGCGA23300.011.68952511
CGCGAGT23100.011.48573713
ACGTGCG25700.011.1416448
CGAGTCG25250.010.50070815
GCATTCG16650.010.2690353
CGCTCGC41200.010.2079170
AACACGT28050.010.20614055
GCACGAA28700.09.791903529
TAACACG27350.09.6987674
CGAACGA9900.09.4733459
CATTCGG19000.09.3686474
CGATAGA16700.09.22040667
CGAAGAT29650.09.057771
GCGCTAA13050.09.03817942
GCGATAG16750.08.93923166
TACGCGA3150.0079561698.8684819
Produced by FastQC (version 0.11.5)