FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17078761
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211450.12380874701625019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG20150.012.91521127
TGCGCGA22700.012.14576511
GTCTAAC24400.011.8602581
CGCACGA23100.011.41644628
CGAGTCG24050.011.30675515
ACGTGCG27450.010.4257978
CGCGAGT25800.010.41311313
AACACGT29350.010.1057935
TAACACG27400.010.058334
CGCTCGC44950.09.47303270
CGCGCTA13350.09.35864546
TACCGGT8505.4569682E-118.98135844
CGACCGT12450.08.961621
CGGGGTT35300.08.8894251
CATTCGG17850.08.7947534
AACGGCG38600.08.71618370
TAACGGC25650.08.60986969
GCACGAA30700.08.59027529
AGTAACG24900.08.57602167
GTAACGG24500.08.46726968
Produced by FastQC (version 0.11.5)